Forum: General
06-06-2015, 01:25 AM
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Replies: 1
Views: 2,359
i was bit early in posting this..:).. found a...
i was bit early in posting this..:).. found a solution myself... if anyone needs this info...
Go to this website
http://www.webact.org/WebACT/generate
upload ur sequences... and in a while...
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Forum: General
06-06-2015, 12:35 AM
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Replies: 1
Views: 2,359
Help in ACT(artemis) comparision tool
Hi,
i have three whole genome sequence from bacteria and i want to compare them. I am trying to Use ACT from artemis for the comparison. While i am trying to upload the files it is prompting me to...
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Forum: Sample Prep / Library Generation
04-14-2015, 06:17 PM
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Replies: 2
Views: 1,475
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Forum: Bioinformatics
01-13-2015, 05:44 PM
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Replies: 0
Views: 962
Draing the WGS mapping
Hi,
I wanna draw a circular genomic map of the bacterial DNA. Unfortunately i have contigs only. Is there any software where i can compare the contigs into reference(circular bacterial DNA) and...
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Forum: Bioinformatics
09-26-2014, 12:33 AM
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Replies: 0
Views: 1,013
Hardware requirement for Meta genomics analysis
Hi guys,
We are planning to perform a meta-genomic analysis using Iontorrent 16s amplicon sequencing.
However, i would like to know what is the decent configuration for the Computer hardware......
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Forum: Bioinformatics
01-11-2014, 12:15 AM
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Replies: 0
Views: 1,133
Whole genome alighment and comparision
Hi,
Needed some advice one whole genome alignment.
I use Mauve for alignment. However when i use the Mauve contig mover it arranges the LCB's similar to reference LCB's....
can anyone tell...
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Forum: Bioinformatics
12-19-2013, 11:59 PM
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Replies: 7
Views: 2,307
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Forum: Bioinformatics
12-19-2013, 05:44 PM
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Replies: 7
Views: 2,307
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Forum: Bioinformatics
12-19-2013, 04:58 AM
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Replies: 7
Views: 2,307
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Forum: Bioinformatics
12-18-2013, 10:42 PM
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Replies: 7
Views: 2,307
Help with annotations
Hi,
I have WGS of Bacteria.
However when i cross checked the annotation done by RAST-SEED server.
i can see some errors.
Same nucleotides have been given different annotation names.
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Forum: Bioinformatics
11-12-2013, 12:41 AM
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Replies: 4
Views: 1,126
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Forum: Bioinformatics
11-12-2013, 12:20 AM
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Replies: 4
Views: 1,126
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Forum: Bioinformatics
11-11-2013, 09:25 PM
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Replies: 4
Views: 1,126
Whole genome alighment
Hi,
Can someone suggest me the tools which can be used for whole genome alignments.
I am having the data in contigs and not as one complete circular genome.
and i need to compare two...
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Forum: Ion Torrent
07-01-2013, 05:24 PM
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Replies: 7
Views: 6,457
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Forum: Ion Torrent
06-26-2013, 08:07 PM
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Replies: 7
Views: 6,457
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Forum: Ion Torrent
06-25-2013, 04:47 PM
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Replies: 7
Views: 6,457
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Forum: Illumina/Solexa
06-25-2013, 04:44 PM
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Replies: 4
Views: 2,258
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Forum: Illumina/Solexa
06-25-2013, 08:03 AM
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Replies: 4
Views: 2,258
Hi,
i am having same concern. though i am...
Hi,
i am having same concern. though i am using Ion torrent for the same. Any idea what is the ideal coverage i need to maintain to get the accurate results...
if u come across any info, please...
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Forum: Ion Torrent
06-25-2013, 07:20 AM
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Replies: 7
Views: 6,457
Ion torrent: bacterial WGS coverage
Hi all,
I am new to ion torrent.
We are interested in getting the bacterial whole genome sequencing( ~2MB )...
However, i am not sure what is the coverage/depth we need to use to get the...
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Forum: Bioinformatics
01-19-2012, 12:14 AM
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Replies: 3
Views: 1,825
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Forum: Bioinformatics
01-19-2012, 12:14 AM
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Replies: 3
Views: 1,825
Hi,
i got the reply from RAST team///i am...
Hi,
i got the reply from RAST team///i am pasting the reply below....
Unfortunately, the only type of promoter sites RAST can recognize
are Ribosomal Binding Sites. RAST cannot yet...
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Forum: Bioinformatics
01-18-2012, 06:39 PM
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Replies: 3
Views: 1,825
annotations
I use annotations pipelines like RAST or NCBI or JGI to get the bacterial genome annotation...
however, if there is any mutation among the promoter of the gene, does the servers annotated them...
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Forum: Bioinformatics
01-18-2012, 06:28 PM
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Replies: 1
Views: 1,367
Also one more question....
However, However,...
Also one more question....
However, However, i have a query about annotation. I have two WGS, one mutant and other wild type.....
while performing the annotation, if there is any mutation in the...
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Forum: Bioinformatics
01-17-2012, 05:13 PM
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Replies: 1
Views: 1,367
annotations intervals problem
Hi guys,
I have two whole genome sequence pf bacteria, mutant and wild type.
I got this annotates in RAST server. and when i try to compare any specific gene and gene product....there is some...
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Forum: Bioinformatics
12-16-2011, 04:31 PM
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Replies: 5
Views: 3,355
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