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Search: Posts Made By: Lucilia
Forum: General 02-07-2012, 05:53 AM
Replies: 6
Views: 8,149
Posted By Lucilia
So... That's what I' m thinking to do: purify...

So... That's what I' m thinking to do: purify more total RNA, considering the degradation problem, and perform another purification step ( invisorb) before mRNA isolation (I'm guessing that some...
Forum: General 02-03-2012, 12:08 PM
Replies: 6
Views: 8,149
Posted By Lucilia
Hi Phillip, Thanks for the considerations. You...

Hi Phillip, Thanks for the considerations.
You are right! The profile is mRNA mode. I also checked the RIN number of the samples and it is bellow 8 (a number that Ive read here would be indicated)....
Forum: General 02-01-2012, 11:19 AM
Replies: 6
Views: 8,149
Posted By Lucilia
Hi Mellissa I have a worse situation than...

Hi Mellissa
I have a worse situation than Nigel. My sequencing results showed 86% of rRNA ( I have to check the parameters used). The service provider showed me the mRNA agilent profile and it...
Forum: Sample Prep / Library Generation 01-10-2012, 04:11 PM
Replies: 22
Views: 11,851
Posted By Lucilia
ribo-zero plants

Do this kit work well for plant material?
Forum: RNA Sequencing 10-27-2011, 12:29 PM
Replies: 3
Views: 2,664
Posted By Lucilia
thanks puendra and Phillip. I have a closely...

thanks puendra and Phillip.
I have a closely related genome available. The service provider used this term "10% per channel". I understood as Phillip explained.
Forum: RNA Sequencing 10-26-2011, 11:07 AM
Replies: 3
Views: 2,664
Posted By Lucilia
RNA-seq depth/plant

Hi everybody!
I'm a beginner in this subject and I have a doubt about the numbers of reads / coverage necessary for plant mRNA sequencing. I would like to sequence mRNA from apical meristems of a...
Showing results 1 to 6 of 6

 


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