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Forum: Illumina/Solexa 01-31-2011, 02:51 AM
Replies: 43
Views: 135,385
Posted By athos
Hi BearClaw, I was wondering what size your...

Hi BearClaw,
I was wondering what size your target region was, and also what percentage of your sequence reads were 'on target'? Sorry if the info is here and I've missed it!
Cheers
Forum: Illumina/Solexa 01-28-2011, 08:05 AM
Replies: 2
Views: 3,954
Posted By athos
Agilent Sure Select

I have a question about the Sure Select Multiplex kit - I notice that they use a different primer to that which is included in the Illumina multiplex sample prep kit. Does anyone know what the...
Forum: Illumina/Solexa 12-07-2009, 03:20 AM
Replies: 6
Views: 5,107
Posted By athos
Hi, thanks for the reply. We're finding that...

Hi, thanks for the reply.

We're finding that the standard Covaris protocol (special tube holder and tubes with beads) for the approx. 3kb fragments is working pretty well (large peak at the...
Forum: Illumina/Solexa 12-04-2009, 08:03 AM
Replies: 6
Views: 5,107
Posted By athos
Mate Pair Library Prep

Has anyone been using this? We've been trying to prepare some libraries with the Illumina kit, and are struggling to get enough DNA through at various stages of the process to do this successfully....
Forum: Illumina/Solexa 07-23-2009, 03:07 AM
Replies: 5
Views: 4,288
Posted By athos
custom sequencing primers

Hi,

Does anyone use their own primers for sequencing on the GAII, i.e rather than the Illumina supplied primer? If so, what purification/modifications do you use? I'm wondering if, for example,...
Forum: Illumina/Solexa 10-15-2008, 06:29 AM
Replies: 1
Views: 1,840
Posted By athos
Question focus uniformity on GAII

Was just wondering if anyone had ever had a situation where the focus uniformity on a GAII was poor for just one base? We have had this problem, where the G values are all significantly lower than...
Forum: Sample Prep / Library Generation 09-21-2008, 11:58 PM
Replies: 19
Views: 21,036
Posted By athos
thanks Chipper. Can I just ask - do you normally...

thanks Chipper. Can I just ask - do you normally follow the Illumina sample prep protocol as is, or do you ever do the size selection after the enrichment PCR?
Cheers.
Forum: Sample Prep / Library Generation 09-19-2008, 01:02 AM
Replies: 19
Views: 21,036
Posted By athos
ChIP seq sample prep for Illumina

Hi Everyone, I'm hoping someone can give some advice on ChIP enriched DNA. Does anyone try and quantify this by nanodrop/picogreen before starting the Illumina sample prep protocol (the protocol...
Forum: Epigenetics 09-19-2008, 12:56 AM
Replies: 2
Views: 4,978
Posted By athos
ChIP seq sample prep for Illumina

Hi Everyone, I'm hoping someone can give some advice on ChIP enriched DNA. Does anyone try and quantify this by nanodrop/picogreen before starting the Illumina sample prep protocol (the protocol...
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