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Forum: Illumina/Solexa 04-25-2017, 06:23 PM
Replies: 4
Views: 1,155
Posted By velu
Thank you. I will go through the possibilities...

Thank you.
I will go through the possibilities you have suggested.
Forum: Illumina/Solexa 04-25-2017, 04:31 AM
Replies: 4
Views: 1,155
Posted By velu
Thank you, Dario1984. Then, what method do you...

Thank you, Dario1984.
Then, what method do you follow for the assembly of reads that do not have such limitations?
Forum: Illumina/Solexa 04-21-2017, 09:05 PM
Replies: 4
Views: 1,155
Posted By velu
with cufflinks, can there be "0" fpkm value and yet full read support?

Hi,
I used hisat2 (2.0.5) to map 150b Paired-end reads on to the draft genome of castorbean and used cufflinks (version 2.2.1) to perform reference annotation based transcript assembly (RABT). This...
Forum: RNA Sequencing 01-17-2017, 08:41 PM
Replies: 0
Views: 1,178
Posted By velu
Post fraction of genes up- and down- regulated

Hi all,
In some perturbed situation (biotic, abiotic stress, etc,), are the fraction of genes up- and down-regulated are similar or can they differ so much that almost all the genes are either up-...
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