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Forum: Bioinformatics 08-07-2018, 07:45 AM
Replies: 665
Views: 150,620
Posted By sunnycqcn
Hello Brian, After running mapPacBio.sh, how...

Hello Brian,
After running mapPacBio.sh, how can I combine the sequence of the same ID?
for example I want to combine the sequences as following:...
Forum: Bioinformatics 08-02-2018, 05:29 AM
Replies: 32
Views: 19,174
Posted By sunnycqcn
It is not working. I used fasta format. Thanks,...

It is not working. I used fasta format.
Thanks,
Fuyou
Forum: Bioinformatics 08-01-2018, 05:47 PM
Replies: 32
Views: 19,174
Posted By sunnycqcn
Hi Brian Bushnell, when I used mapPacBio.sh for...

Hi Brian Bushnell,
when I used mapPacBio.sh for mapping pacbio reads. I met the errors as following:
Exception in thread "Thread-23" java.lang.AssertionError: Read 20, length 10550, exceeds the...
Forum: Bioinformatics 03-08-2016, 03:29 PM
Replies: 252
Views: 93,015
Posted By sunnycqcn
Usually, you can try a small dataset firstly. ...

Usually, you can try a small dataset firstly.
Then software work well, you can run lots of data.
I am sure that my data run well now.
Forum: Bioinformatics 03-08-2016, 03:24 PM
Replies: 252
Views: 93,015
Posted By sunnycqcn
How many libraries is in your libraries files.

How many libraries is in your libraries files.
Forum: Bioinformatics 03-08-2016, 02:58 PM
Replies: 252
Views: 93,015
Posted By sunnycqcn
I am sure the 3.0 version work well with the big...

I am sure the 3.0 version work well with the big genome. I assembled one genome last month, it worked well. Meanwhile, I met the same problem as same with you. So if your library file include a lot...
Forum: Bioinformatics 03-08-2016, 12:16 PM
Replies: 252
Views: 93,015
Posted By sunnycqcn
Could you present your whole command?

Could you present your whole command?
Forum: RNA Sequencing 03-04-2016, 08:42 AM
Replies: 0
Views: 902
Posted By sunnycqcn
Small RNA sequence!

Hi,
why is small RNA sequence alignment with reference genome? Do small RNA sequence read need to transfer to RNA, then alignment with mirBASE?
Thanks,
Fuyou
Forum: Bioinformatics 11-24-2015, 01:02 PM
Replies: 2
Views: 1,918
Posted By sunnycqcn
Did you solve your problem?

I met the same problem. Did you solve your problem?
If you have, could you share your experiences?
Thanks
Forum: Bioinformatics 11-19-2015, 03:48 PM
Replies: 252
Views: 93,015
Posted By sunnycqcn
Did you solve your problem?

______________________________________________
Did you solved your problem?
I met the same problem with you. If you have solved it, could you share it with me?
Thanks,
Fuyou
Forum: Bioinformatics 11-19-2015, 03:42 PM
Replies: 252
Views: 93,015
Posted By sunnycqcn
覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧 Did you...

覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧覧
Did you solved your problem?
I met the same problem with you. If you have solved it, could you share it with me?
Thanks,
Fuyou
Forum: Bioinformatics 09-14-2015, 07:55 AM
Replies: 3
Views: 1,081
Posted By sunnycqcn
Thanks, you are right.

Thanks, you are right.
Forum: Bioinformatics 09-14-2015, 07:18 AM
Replies: 3
Views: 1,081
Posted By sunnycqcn
extract data from txt files

I want to extract gene expression values from a txt file based on gene names.

for example:

A 1500

B 2500

C 3500
Forum: Illumina/Solexa 08-28-2015, 11:57 AM
Replies: 2
Views: 940
Posted By sunnycqcn
Extract the header of illumina sequence!

I have question about our DNA sequence data.
If the format of sequence data is
@HWI-ST1234:136:C5F6VACXX:6:1101:4121:2231 1:N:0:ACTTGA...
Forum: Introductions 05-01-2015, 02:56 PM
Replies: 2
Views: 2,025
Posted By sunnycqcn
I met the same problem.

I met the same problem.
Forum: Bioinformatics 12-04-2014, 01:58 PM
Replies: 3
Views: 3,822
Posted By sunnycqcn
Hi Sir, Did you find why it is now? I have same...

Hi Sir,
Did you find why it is now? I have same problem.
Could you tell me how to do?
Thanks,
Forum: Bioinformatics 12-04-2014, 01:56 PM
Replies: 3
Views: 3,822
Posted By sunnycqcn
Did you find why it is? I have same question.

Did you find why it is?
I have same question.
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