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Forum: Sample Prep / Library Generation 10-11-2016, 08:47 AM
Replies: 2
Views: 909
Posted By sunitha
Thank you very much Manna. I wrote to Illumina. ...

Thank you very much Manna. I wrote to Illumina. I will post the reply from Illumina.

Thanks again
Forum: RNA Sequencing 10-08-2016, 08:52 PM
Replies: 0
Views: 925
Posted By sunitha
for loop for vdb-validate

Hi All

I have an assignment as part of my course work. I am asked to download eight .sra files and then use a for loop command to validate it using vdb-validate. Can anyone help me in writing...
Forum: Sample Prep / Library Generation 10-06-2016, 12:42 PM
Replies: 2
Views: 909
Posted By sunitha
Accidentally left second strand synthesis tube at RT

Hi All

I am making Truseq stranded RNA library. I accidentally left the second strand synthesis tube at room temperature overnight? Will the second strand synthesis reaction work next time.
...
Forum: Sample Prep / Library Generation 08-19-2016, 02:13 PM
Replies: 2
Views: 726
Posted By sunitha
Thank you very much.

Thank you very much.
Forum: Sample Prep / Library Generation 08-18-2016, 01:54 PM
Replies: 2
Views: 726
Posted By sunitha
custom indexed primers for small RNA library

Hi all

I am currently using Illumina kit A and kit B for making small RNA libraries. We need two more indexed primers from kit C to complete our sample set. Could anyone let me know the...
Forum: Sample Prep / Library Generation 12-30-2015, 08:37 AM
Replies: 6
Views: 1,578
Posted By sunitha
Thumbs down Thank you for your reply. I probed the small RNA...

Thank you for your reply. I probed the small RNA purified using miRPremier kit with miR166 probe. I did see hybridization. I had eluted the small RNA in nuclease free water. Right now we do not...
Forum: Sample Prep / Library Generation 12-26-2015, 10:41 AM
Replies: 6
Views: 1,578
Posted By sunitha
Hello All I tried making small RNA library...

Hello All

I tried making small RNA library using small RNA purified using miR Premier kit. I did not succeed in getting amplification. We do not have small RNA chips to do bioanalyzer test. ...
Forum: Sample Prep / Library Generation 12-23-2015, 06:47 AM
Replies: 6
Views: 1,578
Posted By sunitha
Thank you for your reply. I suspected the same...

Thank you for your reply. I suspected the same and probed the total RNA with miR166 and found there is accumulation of small RNA but relatively small amounts. I will take your advise and run a small...
Forum: Sample Prep / Library Generation 12-22-2015, 12:35 PM
Replies: 6
Views: 1,578
Posted By sunitha
Problem in Making Small RNA Library from Grape

Hello All

I am having trouble in amplifying small RNA library using Tru-Seq small RNA kit. I used 1 ug of Total RNA from grape leaf. I had checked the quality using Bioanalyzer (RIN was >8). I...
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