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Forum: Sample Prep / Library Generation 01-29-2019, 07:07 AM
Replies: 17
Views: 8,268
Posted By anineo
Bioanalyzer

Hi again,
have any of you tried to verify that the fragmentation went as it should with Agilent bioanalyzer? To check if the large fragments are actually overamplification?
Forum: Sample Prep / Library Generation 05-31-2018, 02:22 AM
Replies: 17
Views: 8,268
Posted By anineo
Great, how did you fix it? Fewer PCR cycles? ...

Great, how did you fix it? Fewer PCR cycles?

We observed a high duplication rate with FFPE also. Whether it is normal I do not know, but it seems that most studies that use UMIs experience a...
Forum: Sample Prep / Library Generation 05-30-2018, 11:49 PM
Replies: 17
Views: 8,268
Posted By anineo
Hi, we also had a lot of duplication. We...

Hi,
we also had a lot of duplication. We sequenced at 2500x and ended up with a unique depth at around 200x. In our case it may be attributed to low complexity in the sample, as we started with 20...
Forum: Service Providers 11-09-2017, 02:18 AM
Replies: 2
Views: 6,692
Posted By anineo
QIAseq targeted DNA panel for cfDNA analysis

I would like to get in touch with someone who have used QIAseq targeted DNA panel. We are struggling with cfDNA libraries and I am wondering if anyone are experiencing the same?
Forum: Sample Prep / Library Generation 03-20-2017, 01:12 AM
Replies: 17
Views: 8,268
Posted By anineo
Hi, have you gotten any answers from Qiagen?...

Hi,
have you gotten any answers from Qiagen? Or solved the problem?
We have constructed libraries from FFPE DNA and experience the same problems, lots of fragments around 1000 bp.

(The...
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