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Forum: General 04-05-2018, 10:15 AM
Replies: 0
Views: 700
Posted By scrosby
ABRF NGS Side Meeting 4/24

At ABRF 2018 Meeting in Myrtle Beach (https://conf.abrf.org/), there will be an ‘invitation only’ evening discussion on issues affecting Next Generation Sequencing cores. This survey...
Forum: RNA Sequencing 03-09-2018, 05:27 AM
Replies: 2
Views: 1,879
Posted By scrosby
260 views...NO replies!! Maybe a more extensive...

260 views...NO replies!!
Maybe a more extensive search would have revealed nothing!
Forum: RNA Sequencing 03-02-2018, 07:47 AM
Replies: 2
Views: 1,879
Posted By scrosby
RNA-seq in the NovaSeq

The smallest S1 kit is 100-cycles. With RNA-seq this results in a choice of single-end (100bp) or paired-end (2x50).

For the detection of splice junctions in, say, 300bp fragments...


Perhaps...
Forum: Academic/Non-Profit Jobs 07-07-2016, 04:02 PM
Replies: 0
Views: 629
Posted By scrosby
St. Louis, Washington University

Job Type/Schedule
This opportunity is full-time and works approximately 40 hours per week. Hours are generally normal business hours but may vary due to the timing for experiments and other research...
Forum: RNA Sequencing 04-12-2016, 03:45 AM
Replies: 3
Views: 1,196
Posted By scrosby
80% ethanol

Thanks to both of you?

These are shipped at room temp?
Is methylnick's concern about shipping a flammable a concern?
Forum: RNA Sequencing 04-07-2016, 12:10 PM
Replies: 3
Views: 1,196
Posted By scrosby
Ship RNA @ RT in isoprop?

I have been asked a few times and have not been able to find a clear answer online. Is there any reason that RNA pellets cannot be shipped at room temp in 100% isopropanol?
Forum: Academic/Non-Profit Jobs 08-26-2015, 06:55 AM
Replies: 0
Views: 406
Posted By scrosby
Meet me in St. Louis! (WashU: Tech II)

Job Type/Schedule
This position is full-time and works approximately 40 hours per week.

Department Name/Job Location
This position is for the Genome Technology Access Center for the Medical...
Forum: Illumina/Solexa 03-18-2015, 05:11 AM
Replies: 10
Views: 3,468
Posted By scrosby
Checked BaseSpace already. Just 2x76.

Checked BaseSpace already. Just 2x76.
Forum: Illumina/Solexa 03-17-2015, 01:16 PM
Replies: 10
Views: 3,468
Posted By scrosby
HiSeq 3000/4000 2x150 data

Hello wise ones,

Has anyone out there had any paired-end long read experience. We'd love to see the run data (Q30 graphs, etc).

S
Forum: Bioinformatics 01-17-2014, 04:44 AM
Replies: 4
Views: 1,027
Posted By scrosby
I made this. Does it look right? ...

I made this. Does it look right?

http://i.imgur.com/mXjlwTU.png

It suggests that to get >95% 30-fold coverage I need an average coverage of 41...can that be right??
Forum: Bioinformatics 01-16-2014, 09:36 AM
Replies: 4
Views: 1,027
Posted By scrosby
Advanced coverage predictor

Hi all,

Is there some kind of a Poisson calculator out there that will predict not only mean coverage but coverage at various percents? For example, given a human genome, what mean coverage do I...
Forum: Sample Prep / Library Generation 01-30-2013, 06:17 AM
Replies: 1
Views: 2,840
Posted By scrosby
Pre-capture

We are looking at this pool as well, but have not yet made the decision to dive in (har-har). Has anyone had significant experience with pre-capture pooling?
Forum: Academic/Non-Profit Jobs 11-08-2012, 09:47 AM
Replies: 0
Views: 654
Posted By scrosby
Computational Biologist, Washington Univ.

Job Description
The Genome Technology Access Center (https://gtac.wustl.edu/) at Washington University is looking for a computational biologist with several years of experience to work closely with...
Forum: Core Facilities 10-07-2011, 08:01 AM
Replies: 1
Views: 1,777
Posted By scrosby
Need to Outsource

Hi all,

While we at GTAC have increased our capacity, we sometimes have more NGS than we can get done in a timely manner. Would you consider taking on some out-source work? If so could you...
Forum: Illumina/Solexa 10-05-2011, 04:13 PM
Replies: 5
Views: 2,518
Posted By scrosby
Just got off the phone with Illumina. dhinerfeld...

Just got off the phone with Illumina. dhinerfeld is spot-on. The room temp must always be 22 +/- 3 and cannot exceed +/-2 during a run. And that does NOT mean it's ok for the temp to swing above...
Forum: Events / Conferences 10-05-2011, 11:19 AM
Replies: 0
Views: 1,573
Posted By scrosby
Midwest Association of Core Directors, Chicago, 10/27

October 27-30, Chicago
Midwest Association of Core Directors Second Annual Meeting

Agenda (http://www.mwacd.org/node/29)

Keynote Speakers:
Mark Lively, Wake Forest University
Steve Bobin,...
Forum: RNA Sequencing 09-15-2011, 03:11 AM
Replies: 5
Views: 8,040
Posted By scrosby
GTAC at WashU

Hello,

We provide, among other services, RNA-seq, with free analysis. Our prices can be seen here:
http://gtac.wustl.edu/pricing.php

Seth

__________________________________________
Seth...
Forum: Illumina/Solexa 09-13-2011, 08:31 AM
Replies: 0
Views: 1,370
Posted By scrosby
Complete vs Illumina

Has there been any agnostic comparison done between the genome sequencing services of Complete Genomics vs. Illumina Genome Network (cost, data, turn-around time, etc)?
Forum: 454 Pyrosequencing 09-11-2011, 07:58 AM
Replies: 12
Views: 37,292
Posted By scrosby
Forgive me...

I should have said 'soon' going to to 150 PE. Before the end of the year, I believe?
Forum: Helicos / Direct Genomics 09-11-2011, 07:43 AM
Replies: 19
Views: 37,623
Posted By scrosby
genseq, I like the horse-race! You should update...

genseq, I like the horse-race! You should update it!
Forum: 454 Pyrosequencing 09-11-2011, 07:34 AM
Replies: 2
Views: 2,167
Posted By scrosby
Forum: 454 Pyrosequencing 09-11-2011, 07:30 AM
Replies: 12
Views: 37,292
Posted By scrosby
With Illumina now going to 300bp, does there...

With Illumina now going to 300bp, does there remain a viable role for 454?
Forum: Academic/Non-Profit Jobs 05-27-2011, 01:07 PM
Replies: 0
Views: 1,093
Posted By scrosby
Computational Biologist (St. Louis)

Job Description
The Washington University Genome Technology Access Center (GTAC.wustl.edu) is recruiting a clinical computational biologist for the newly emerging Genetic Pathology Services at...
Forum: General 05-04-2011, 07:36 AM
Replies: 66
Views: 65,943
Posted By scrosby
Gtac

This is stellar work! Could you please add:

The Genome Technology Access Center at Washington University
Campus Box 8232
660 South Euclid
St. Louis, MO 63110
314.286.1256

We currently...
Forum: Bioinformatics 04-02-2010, 08:10 AM
Replies: 3
Views: 1,970
Posted By scrosby
Looking for LIMS

Rather than putting the resources into building one, we are seeking to buy or borrow a LIMS. Our facility will be NextGen (2 HiSeqs, 2-3 GAIIx) and currently has a complete (Affy, Agilent, illumina,...
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