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Forum: Illumina/Solexa 10-13-2016, 06:39 AM
Replies: 16
Views: 4,436
Posted By DRYTCYV
Hi everyone, Thank you huguesparri for...

Hi everyone,



Thank you huguesparri for your input!

We had some meetings and my last informations are:

- Epmotion 5075 : The software looks easy to work, I thought it was more difficult....
Forum: Illumina/Solexa 10-05-2016, 05:44 AM
Replies: 16
Views: 4,436
Posted By DRYTCYV
Thank you huguesparri for your reply, it was very...

Thank you huguesparri for your reply, it was very useful. Do you use official protocols or you design your owns?

For RNAseq we are thinking to use this one :...
Forum: Illumina/Solexa 10-04-2016, 05:41 AM
Replies: 16
Views: 4,436
Posted By DRYTCYV
Hi, Our list just increase with 1 more...

Hi,

Our list just increase with 1 more brand, but we still have some questions/concerns that we're trying to get in contact with the tech support.

- Epmotion 5075 : In the beginning, they said...
Forum: Illumina/Solexa 09-30-2016, 06:27 AM
Replies: 16
Views: 4,436
Posted By DRYTCYV
Hello everyone, Does anyone have more...

Hello everyone,

Does anyone have more information to update this post?

We're in the middle of the same process to choose a Liquid Handling Robot, and our list is:

- Hamilton NGS

-...
Forum: Illumina/Solexa 05-06-2016, 10:16 AM
Replies: 5
Views: 1,107
Posted By DRYTCYV
The replacement for this kit arrived today ...

The replacement for this kit arrived today
We'll receive another kit, from other Run, no more replacements because of the high cluster density :D

This kit its very stable even in overclustering...
Forum: Illumina/Solexa 05-06-2016, 09:47 AM
Replies: 5
Views: 1,107
Posted By DRYTCYV
You're completely right :D Lib prep :...

You're completely right :D

Lib prep : Nextera XT
Samples: Mixed genomes no Phix
Forum: Illumina/Solexa 05-06-2016, 09:32 AM
Replies: 5
Views: 1,107
Posted By DRYTCYV
Miseq V2 kit (2x250 cycles) drop quality

Hi,

We detected some drop quality (R2) on the Miseq V2 kit (2x250 cycles), anyone else?
p. ex. R1 finished arround 87% Q30 and R2 with 67%.

Is the V2 kit the new (old) V3 (2x300 cycles)?
...
Forum: Illumina/Solexa 04-08-2016, 01:55 AM
Replies: 68
Views: 19,974
Posted By DRYTCYV
I got one of that cartridges :) Our FAS said...

I got one of that cartridges :)

Our FAS said that Illumina wants to release "the new" V3 kit after Summer.
The last V3 kit we used as a V2 2x250 cycles, the difference is that the V3 got more...
Forum: Illumina/Solexa 12-22-2015, 03:53 AM
Replies: 4
Views: 1,606
Posted By DRYTCYV
MiSeq Run started: 20:30 (Friday) Error msg :...

MiSeq Run started: 20:30 (Friday)
Error msg : 21:30

Who to call?

A) Illumina techsupport
B) SEQanswers amazing tech support (and sometimes the only tech support)
C) Ghostbusters

The...
Forum: Illumina/Solexa 12-18-2015, 04:03 PM
Replies: 4
Views: 1,606
Posted By DRYTCYV
MiSeq Failed Run

Hello,

I got this:

"Your run xxxxxxxx on instrument xxxxxxx recently encountered the following error during sequencing:

<Internal Illumina Command Exception>"

Any ideas?
Forum: Illumina/Solexa 11-12-2015, 02:27 AM
Replies: 68
Views: 19,974
Posted By DRYTCYV
My idea is that the missing information about the...

My idea is that the missing information about the quality problem it's a little bit naive for a big company like Illumina? I'm just saying this because its impossible to change something in a...
Forum: Core Facilities 10-14-2015, 05:53 AM
Replies: 4
Views: 4,151
Posted By DRYTCYV
Is there any liquid handling users that can give...

Is there any liquid handling users that can give some feedback?
Its for a genomic core facility with a Illumina platform technology (Miseq, NextSeq or HiSeq).

epMotion 5075 vs Hamilton STAR vs...
Forum: Illumina/Solexa 07-09-2015, 01:27 AM
Replies: 68
Views: 19,974
Posted By DRYTCYV
Yes, same thing here

Yes, same thing here
Forum: Illumina/Solexa 06-29-2015, 04:06 PM
Replies: 68
Views: 19,974
Posted By DRYTCYV
Hello everybody, The quality drop its a real...

Hello everybody,

The quality drop its a real issue, we already report to Illumina 4-5 Runs, with more or less the same metrics (last one R2=52%Q30). We got cluster density from 900 to 1400 k/mm2...
Forum: Sample Prep / Library Generation 05-28-2015, 09:01 AM
Replies: 17
Views: 5,011
Posted By DRYTCYV
Hi, Anyone tried KAPA HyperPlus Kits? They...

Hi,

Anyone tried KAPA HyperPlus Kits?
They say that this is the direct nextera competitor.

Cheers,
Forum: Illumina/Solexa 05-28-2015, 08:49 AM
Replies: 16
Views: 4,589
Posted By DRYTCYV
Hi Vinz, Thank you for your post. It was...

Hi Vinz,

Thank you for your post.
It was me that opened this discussion about the contamination, just because we detected some biofilm in the manual sipper. And after the new non-official bleach...
Forum: Illumina/Solexa 05-05-2015, 04:15 PM
Replies: 16
Views: 4,589
Posted By DRYTCYV
Hi everyone, sorry for the late reply and I need...

Hi everyone, sorry for the late reply and I need more time to check the previous Runs metrics.


I contacted tech support and they confirmed that the fungal contamination could be causing the...
Forum: Illumina/Solexa 04-21-2015, 05:38 AM
Replies: 16
Views: 4,589
Posted By DRYTCYV
Hi nangel, Thank you for your reply ...

Hi nangel,

Thank you for your reply



Now there´s some metrics that make sense, the prephasing increase, Q scores problems etc. I'm gona check the last runs to confirm this.

Now the...
Forum: Illumina/Solexa 04-15-2015, 07:56 AM
Replies: 16
Views: 4,589
Posted By DRYTCYV
MiSeq fungal contamination

Hello,
We detected some kind of biofilm on the Miseq manual sipper and have reported to Illumina support. The reply from the supposed Illumina support was a little bit unsupported. They said that...
Forum: Illumina/Solexa 09-14-2014, 10:31 AM
Replies: 55
Views: 28,764
Posted By DRYTCYV
Is this what you are looking for? ...

Is this what you are looking for?

http://supportres.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf
Forum: Illumina/Solexa 07-22-2014, 02:11 AM
Replies: 2
Views: 1,473
Posted By DRYTCYV
Hi, Do you know the fragment size?

Hi,

Do you know the fragment size?
Forum: Illumina/Solexa 07-15-2014, 08:15 AM
Replies: 3
Views: 2,028
Posted By DRYTCYV
Hi dfhdfh, Thank you for your feedback. We...

Hi dfhdfh,

Thank you for your feedback.
We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the...
Forum: Illumina/Solexa 07-13-2014, 09:37 AM
Replies: 3
Views: 2,028
Posted By DRYTCYV
Bubbles in Miseq Flowcell

Hi everyone,

I would like to know your opinion about the images in attachment.
The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble.
I...
Forum: Sample Prep / Library Generation 07-12-2014, 06:52 AM
Replies: 15
Views: 11,381
Posted By DRYTCYV
In that case its a complete different scenario...

In that case its a complete different scenario for this protocol
Forum: Sample Prep / Library Generation 07-11-2014, 03:31 PM
Replies: 15
Views: 11,381
Posted By DRYTCYV
Hi, If you got fragments bigger than 300-400bp,...

Hi,
If you got fragments bigger than 300-400bp, i'll say to give a shot!
The transposase need a minimum of +-300bp to cut and add adaptores, so in some of your fragments will be possible.
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