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Forum: Bioinformatics 09-24-2014, 01:28 AM
Replies: 4
Views: 1,391
Posted By Jegar
I don't have a lot of experience with the...

I don't have a lot of experience with the software mentioned, but I want to put in my two cents on error correction and quality filtering generally.
These two things are usually considered separate...
Forum: Bioinformatics 09-23-2014, 05:40 AM
Replies: 6
Views: 3,788
Posted By Jegar
How you combine these scores depends on the...

How you combine these scores depends on the platform you are using, as the Phred scores are calculated differently.

If they are Illumina scores, I believe it is appropriate to add the scores...
Forum: Bioinformatics 09-23-2014, 04:48 AM
Replies: 15
Views: 19,460
Posted By Jegar
That's all that is needed - thank you for...

That's all that is needed - thank you for answering the question.

The literature around alignment and mapping uses a lot of terms interchangably, so in my mind this is a fair question. You might...
Forum: Bioinformatics 08-06-2014, 01:22 AM
Replies: 17
Views: 9,264
Posted By Jegar
Thanks for your reply and helpful clarification. ...

Thanks for your reply and helpful clarification.

It sounds like a comparison of simulators might be good for a blog post - shame that a helpful piece of work like that wouldn't get published (I...
Forum: Bioinformatics 08-06-2014, 12:33 AM
Replies: 17
Views: 9,264
Posted By Jegar
There seems to be a broad range of RNA-Seq...

There seems to be a broad range of RNA-Seq simulators. Has anyone done a comparison, or know of a paper that examines them empirically? I'd just be interested to know how their different features...
Forum: Introductions 08-04-2014, 08:18 AM
Replies: 0
Views: 984
Posted By Jegar
Thumbs up Bayesian Statistician in Bioinformatics

Hey SEQ users,

This is my first post in this forum, so though I'd better say hello.

I am a Bioinformatic Officer at a company in Cambridge having recently gained by PhD in Bayesian Statistics...
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