SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 9 of 9
Search took 0.00 seconds.
Search: Posts Made By: LSAD
Forum: Core Facilities 03-14-2017, 08:04 PM
Replies: 10
Views: 8,533
Posted By LSAD
Our TruSeq Small RNA library works very well on...

Our TruSeq Small RNA library works very well on HiSeq 4000. It is not listed as compatible with HiSeq4000 on Illumina website because the protocol is prone to produce adapter dimer. Check bioanalyzer...
Forum: Illumina/Solexa 10-29-2012, 05:44 PM
Replies: 43
Views: 28,561
Posted By LSAD
Hi ZAAB, have you used any custom sequencing...

Hi ZAAB, have you used any custom sequencing primers that largely overlap with illumina p5/p7 adapters?
Forum: Illumina/Solexa 10-29-2012, 04:38 PM
Replies: 37
Views: 42,064
Posted By LSAD
We encountered similar problem before (clusters...

We encountered similar problem before (clusters on all channels) when we used sequencing primers annealing to P5/P7. Then we fixed the issue by using primers with similar Tm but annealing to other...
Forum: Illumina/Solexa 06-13-2012, 04:11 PM
Replies: 37
Views: 42,064
Posted By LSAD
Yepler, Except the few nucleotides mentioned...

Yepler,

Except the few nucleotides mentioned earlier by skosuri, another reason of failed run for us is that we used sequencing primer targeting complementary to P5. We tried a different...
Forum: Illumina/Solexa 04-18-2012, 02:45 PM
Replies: 37
Views: 42,064
Posted By LSAD
Thank you a lot for your reply. The Tm problem is...

Thank you a lot for your reply. The Tm problem is highly probable. We have been using this home-brewed library and P5 sequence for sequencing on HiSeq single run kit with no problem. Do you think...
Forum: Sample Prep / Library Generation 04-17-2012, 11:49 PM
Replies: 16
Views: 5,508
Posted By LSAD
When we use our indexed primer, the cluster...

When we use our indexed primer, the cluster formation is not succesful.
Do you happen to know why it is like that?
Forum: Illumina/Solexa 04-17-2012, 11:33 PM
Replies: 37
Views: 42,064
Posted By LSAD
We thought that was the only problem for us but...

We thought that was the only problem for us but now it seems not. It is great to know that you solved your problem, so that I know I am on the right track. It seems that after adding the 6 nt onto...
Forum: Sample Prep / Library Generation 04-13-2012, 01:09 AM
Replies: 0
Views: 2,113
Posted By LSAD
Blocking oligos in MiSeq kit

Does anybody know what are the sequences of blocking oligos provided in HT1 in MiSeq reagent kit?
Forum: Illumina/Solexa 04-13-2012, 12:29 AM
Replies: 37
Views: 42,064
Posted By LSAD
I am interested to know whether the new P5/P7...

I am interested to know whether the new P5/P7 solved your previous problem, because I have been experiencing the same problem. I added 6nt on old P5 and 2nt on old P7 based on Nature 2008 paper. I...
Showing results 1 to 9 of 9

 


All times are GMT -8. The time now is 03:10 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO