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Forum: Bioinformatics 05-25-2017, 10:55 AM
Replies: 7
Views: 1,291
Posted By jmartin
It looks like the variation between quasispecies...

It looks like the variation between quasispecies is making it difficult for tadpole to accomplish what I need, which is a sort of 'central' consensus amongst all these quasispecies which can serve as...
Forum: Bioinformatics 05-19-2017, 08:40 AM
Replies: 7
Views: 1,291
Posted By jmartin
Thanks Brian, I'll try playing a bit more. I'll...

Thanks Brian, I'll try playing a bit more. I'll try using tadpole's error correction too in case it deals with cases that I haven't already corrected.
Forum: Bioinformatics 05-18-2017, 05:27 PM
Replies: 7
Views: 1,291
Posted By jmartin
Thanks for the reply! I went and tried Tadpole...

Thanks for the reply! I went and tried Tadpole and I'm trying various things to fine tune the assembly. One thing I'm wondering is if there is a way to do a reference guided assembly in Tadpole? ...
Forum: Bioinformatics 05-17-2017, 05:46 PM
Replies: 7
Views: 1,291
Posted By jmartin
Best way to build consensus of short reads spanning viral gene

I have a collection of Illumina HiSeq 2000 reads that should span a specific coding region in a viral genome. The region these reads cover is 2625bp. What I want to do is generate a consensus of...
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