Forum: Vendor Forum
04-07-2011, 05:59 AM
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Replies: 10
Views: 9,846
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Forum: Vendor Forum
04-03-2011, 07:14 PM
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Replies: 10
Views: 9,846
barcodes with PCR free prep
We are very interested in amplification free library prep. We are currently using your Nextflex library prep kit. Can we still use all 48 Nextflex barcodes with the the new amplification free...
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Forum: Illumina/Solexa
08-30-2010, 06:11 PM
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Replies: 7
Views: 23,426
in terms of ligation based barcode bias it does...
in terms of ligation based barcode bias it does matter where the barcode is. Adding the barcode to the 5' adapter is not a good idea because the RNA ligase 1 enzyme at this end will prefer some bases...
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Forum: Bioinformatics
08-12-2010, 07:17 PM
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Replies: 12
Views: 4,056
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Forum: Metagenomics
08-12-2010, 07:09 PM
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Replies: 1
Views: 2,228
filtering samples
has anyone used any other method for extracting dna samples from liquid environmental samples other than filters ?
how about soil?
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Forum: Sample Prep / Library Generation
08-12-2010, 07:08 PM
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Replies: 1
Views: 2,725
mag beads?
any recommendations on oligo dT magnetic beads for mRNA pulldown?
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Forum: Sample Prep / Library Generation
08-12-2010, 06:58 PM
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Replies: 0
Views: 2,320
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Forum: Illumina/Solexa
08-12-2010, 06:56 PM
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Replies: 3
Views: 5,425
sounds like your sample might be lacking small...
sounds like your sample might be lacking small RNA? I would recommend running your sample on a small agilent chip:
http://www.chem.agilent.com/Library/usermanuals/Public/G2938-90092_QSG_smallRNA.pdf...
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Forum: Illumina/Solexa
08-09-2010, 07:36 PM
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Replies: 7
Views: 3,661
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Forum: Illumina/Solexa
08-09-2010, 07:25 PM
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Replies: 7
Views: 23,426
multiplex small RNAs
We are multiplexing an average of 8 microRNAs per lane using this strategy :
...
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Forum: Illumina/Solexa
08-04-2010, 08:46 PM
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Replies: 4
Views: 3,778
barcoding small RNAs
we tried barcoding the 5'RNA adapter and found significant bias in our small RNA preps. it turns out that RNA Lig I prefers some bases over others. You will need to barcode the 3' adenylated adapter...
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Forum: Illumina/Solexa
07-27-2010, 07:37 PM
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Replies: 3
Views: 2,015
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Forum: Illumina/Solexa
07-27-2010, 07:34 PM
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Replies: 19
Views: 12,538
small mirna protocol
westlook, not sure what that sequence is....screened my results, haven't seen it.
for the other folks in the forum, I've gotten the miRNA protocol to work consistently now (30th library today)....
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Forum: Illumina/Solexa
06-15-2010, 06:48 PM
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Replies: 7
Views: 3,661
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Forum: RNA Sequencing
06-15-2010, 06:14 PM
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Replies: 4
Views: 3,095
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Forum: Illumina/Solexa
06-13-2010, 08:18 AM
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Replies: 10
Views: 9,815
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Forum: RNA Sequencing
06-13-2010, 08:16 AM
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Replies: 4
Views: 3,095
We've been using the Bioo AIR small RNA Seq kit...
We've been using the Bioo AIR small RNA Seq kit and have been very happy with it. Significantly less adapter dimers in our sequence runs with this kit compared to v1.5, not to mentioned that the Bioo...
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Forum: Illumina/Solexa
06-12-2010, 07:35 PM
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Replies: 3
Views: 2,260
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Forum: Illumina/Solexa
06-12-2010, 07:33 PM
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Replies: 10
Views: 9,815
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Forum: Illumina/Solexa
06-12-2010, 07:30 PM
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Replies: 28
Views: 13,099
advantages to mRNA seq
Does anyone have any results or new results using this protocol...
Any other advantages of this than regular mRNA seq?
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Forum: Illumina/Solexa
05-27-2010, 05:42 PM
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Replies: 7
Views: 8,444
hi,
the 3' adapter sequence for small RNA...
hi,
the 3' adapter sequence for small RNA sequencing, v1.5 is ATCTCGTATGCCGTCTTCTGCTTG. You'll need it to be 5'adenylated and 3'ddC blocked. You are right the 3' ligation does not require ATP and...
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