Forum: Illumina/Solexa
06-08-2018, 01:02 PM
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Replies: 30
Views: 10,162
Check the concentration of PhiX by qPCR (e.g....
Check the concentration of PhiX by qPCR (e.g. KAPA). I don't think I've yet seen a tube from Illumina come in at 10 nM. It's usually much higher, in my experience.
Also, I've noticed that if you...
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Forum: Sample Prep / Library Generation
06-01-2018, 01:15 PM
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Replies: 1
Views: 2,455
You'll capture DNA adjacent to one-side of the...
You'll capture DNA adjacent to one-side of the oligo integration site whichever direction you choose to use. We went with the negative strand primer as GSP1(-) will have a longer contact with the ODN...
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Forum: Core Facilities
07-26-2017, 02:10 PM
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Replies: 5
Views: 9,074
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Forum: Illumina/Solexa
07-21-2017, 12:31 PM
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Replies: 13
Views: 5,610
I had contacted Illumina - and Sigma - about...
I had contacted Illumina - and Sigma - about alternatives. Sigma says there are none. They were completely unhelpful. They literally told me to just search online for alternatives. (Yeah, no s&!t,...
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Forum: Illumina/Solexa
07-21-2017, 09:09 AM
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Replies: 13
Views: 5,610
I called Sigma. If you can't order any of the...
I called Sigma. If you can't order any of the ProClin products they won't approve an order for SeqClin since it contains ProClin.
Can't find any alternatives for ProClin other than azide. Anyone...
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Forum: Illumina/Solexa
07-19-2017, 11:46 AM
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Replies: 13
Views: 5,610
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Forum: Core Facilities
07-18-2017, 02:43 PM
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Replies: 5
Views: 9,074
How long can the reagents sit in the machine...
How long can the reagents sit in the machine between runs?
I'm interested in suggesting my group buy a FA. We already have a Bioanalyzer 2100 but the kits are expensive and the gel only is good...
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Forum: Illumina/Solexa
03-04-2015, 03:16 PM
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Replies: 14
Views: 14,296
I like the idea that small fragments would be...
I like the idea that small fragments would be denatured, but wouldn't they reanneal? Plus they'd have to probably be at least ~40 bp in length since small fragments should have been removed in the...
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Forum: Illumina/Solexa
03-03-2015, 12:14 PM
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Replies: 14
Views: 14,296
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Forum: Illumina/Solexa
03-03-2015, 11:15 AM
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Replies: 14
Views: 14,296
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Forum: Sample Prep / Library Generation
02-02-2015, 02:00 PM
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Replies: 13
Views: 7,985
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Forum: Bioinformatics
08-18-2014, 11:01 AM
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Replies: 132
Views: 55,990
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Forum: Bioinformatics
08-14-2014, 04:23 PM
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Replies: 132
Views: 55,990
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Forum: Bioinformatics
07-29-2014, 11:34 AM
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Replies: 6
Views: 3,039
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Forum: Illumina/Solexa
05-19-2014, 03:48 PM
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Replies: 10
Views: 5,959
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Forum: Sample Prep / Library Generation
10-17-2013, 11:32 PM
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Replies: 46
Views: 24,331
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Forum: Sample Prep / Library Generation
09-22-2013, 01:01 AM
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Replies: 2
Views: 2,158
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Forum: Sample Prep / Library Generation
05-16-2013, 04:04 PM
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Replies: 46
Views: 24,331
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Forum: Illumina/Solexa
03-13-2013, 02:28 PM
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Replies: 76
Views: 44,952
He's using both. The 100 bp ladder was what he...
He's using both. The 100 bp ladder was what he was size-selecting (to illustrate the use of the SPRI bead ratios), the 1kb+ ladder is the size marker for the gel.
Take a look again at the text...
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Forum: Bioinformatics
02-22-2013, 01:32 PM
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Replies: 10
Views: 12,045
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Forum: Bioinformatics
02-19-2013, 11:00 PM
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Replies: 11
Views: 10,025
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Forum: Bioinformatics
02-19-2013, 06:28 PM
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Replies: 11
Views: 10,025
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Forum: Bioinformatics
02-19-2013, 05:25 PM
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Replies: 11
Views: 10,025
With the H=111 and $H I think arun is just...
With the H=111 and $H I think arun is just setting an awk variable. I'm not sure what use it has actually in this case. The exact same result can be achieved with "...$1,111,$2}..." The 5th column in...
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Forum: Bioinformatics
01-30-2013, 12:44 PM
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Replies: 5
Views: 1,297
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Forum: Bioinformatics
01-30-2013, 12:21 PM
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Replies: 5
Views: 1,297
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