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Forum: Oxford Nanopore 07-06-2017, 05:41 PM
Replies: 2
Views: 3,345
Posted By chen
Oh, that make sence. Thank you very much.

Oh, that make sence.

Thank you very much.
Forum: Oxford Nanopore 07-02-2017, 09:39 PM
Replies: 2
Views: 3,345
Posted By chen
About new starter pack log in ID

Hi,all

We've already bought the new starter pack of the oxford nanopore, but cannot find the nanopore community log in ID from what we've got. we already searched all packages, but got...
Forum: Sample Prep / Library Generation 04-01-2014, 05:48 PM
Replies: 4
Views: 1,738
Posted By chen
yeah. yet this was our first try ChIP-seq...

yeah. yet this was our first try ChIP-seq library, so we just stick to the protocol(Truseq Nano DNA kit). we may improve the ligation step next time.

we would take the smear library to do a test...
Forum: Sample Prep / Library Generation 04-01-2014, 12:01 AM
Replies: 4
Views: 1,738
Posted By chen
Hi zwang, we had a similar result starting...

Hi zwang,

we had a similar result starting with ChIP-DNA, using the Truseq nano pre kit. And we find some smear above the dimers when we redo the PCR step, adding the cycles up to 30 comparing...
Forum: Illumina/Solexa 12-11-2013, 09:45 PM
Replies: 44
Views: 20,407
Posted By chen
We've got similar issue with the Miseq, low...

We've got similar issue with the Miseq, low density at 122K with 0.2N NaOH according to Miseq user guide. It's a polyA mRNA lib using the NEB kit, with 750bp length on average. The final...
Forum: Illumina/Solexa 06-28-2013, 05:08 PM
Replies: 9
Views: 4,266
Posted By chen
hi Genohub, we finally got the sanger...

hi Genohub,

we finally got the sanger sequencing of the library(the lower band) and the upper band, and yes, the library(lower band) is right one(small RNA). the upper band is more like the...
Forum: Illumina/Solexa 06-17-2013, 09:58 PM
Replies: 9
Views: 4,266
Posted By chen
we also try agarose gel electrophoresis on the...

we also try agarose gel electrophoresis on the final step, and cut the right size band(~150bp), and still the 2200 result showed ~130bp. Maybe it's just the deviation of the instrument(like ~20bp...
Forum: Illumina/Solexa 06-16-2013, 05:50 PM
Replies: 9
Views: 4,266
Posted By chen
hi Genohub, Thank you very much for your...

hi Genohub,
Thank you very much for your quick reply!

We used 1ug of total RNA of the human sample, and followed the exact protocol of TruSeq small RNA kit(did not isolate samll RNA from...
Forum: Illumina/Solexa 06-14-2013, 06:50 PM
Replies: 9
Views: 4,266
Posted By chen
small RNA Lib, strange bands, need help

hi, I havre constructed the small RNA library following the exact protocol of ILMN TruSeq small RNA with ILMN small RNA kit. and excised the bands between 145bp and 160bp, but the agilent 2200 showed...
Forum: Sample Prep / Library Generation 04-15-2010, 10:04 PM
Replies: 3
Views: 2,385
Posted By chen
Post Mate pair, high GC

It shows that high GC samples may have a lower concentration after PCR.
Is there any notes to take when preparing the mate pair library with the HIGH GC samples, except the PCR process(increase the...
Forum: Illumina/Solexa 09-06-2009, 11:21 PM
Replies: 18
Views: 14,775
Posted By chen
I used glass tube with 3mg beads in it, and...

I used glass tube with 3mg beads in it, and putting the whole mix into 300ul with TE. The concentration of DNA would be <5ug.

I had tried to lower the beads, and that worked better.

Thanks,...
Forum: Illumina/Solexa 09-05-2009, 09:02 PM
Replies: 18
Views: 14,775
Posted By chen
Thank you, hamid. In our lab, usually we...

Thank you, hamid.

In our lab, usually we use Nebulizer(the FIRST fragment) for shearing of DNA fragments to 2-5Kb, and Covaris for fragmenting the Circularized DNA(the SECOND fragment) to about...
Forum: Illumina/Solexa 09-05-2009, 12:31 AM
Replies: 18
Views: 14,775
Posted By chen
Question Hi everyone, Here I have some questions with...

Hi everyone,
Here I have some questions with the Covaris.
The problems of the stability of Covaris really bother me during the second fragement when I am preparing 2-5Kb samples for MATE PAIR...
Forum: Introductions 09-04-2009, 06:32 PM
Replies: 373
Views: 140,373
Posted By chen
Hi, I'm chen, from shenzhen,China. Now I am...

Hi,
I'm chen, from shenzhen,China. Now I am mostly working the experimental part of the solexa libraries. I am glad to join the community and hope we can help each other.

Besh wishes!
chen
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