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Forum: Illumina/Solexa 01-23-2020, 10:02 AM
Replies: 3
Views: 1,559
Posted By seqsuave
I have heard through the grapevine that 10x...

I have heard through the grapevine that 10x Genomics will be switiching to dual index for all their kits down the road. The new Visium kit has 10bp dual index, and the ATACseq kit already uses dual...
Forum: General 07-15-2019, 02:49 PM
Replies: 3
Views: 5,615
Posted By seqsuave
Has anyone tried half-reactions for the QuantSeq...

Has anyone tried half-reactions for the QuantSeq chemistry?
Forum: Sample Prep / Library Generation 05-23-2019, 01:47 PM
Replies: 12
Views: 2,077
Posted By seqsuave
How fresh was the 80% Ethanol, if ethanol...

How fresh was the 80% Ethanol, if ethanol evaporated off you could have had elution during the washes. It is recommended that 80% Ethanol is not more than a week old.
Forum: Sample Prep / Library Generation 05-22-2019, 01:48 PM
Replies: 0
Views: 923
Posted By seqsuave
10x Genomics Single-cell Half Reactions

Has anyone attempted or successfully done half reactions for 10x Genomics platforms?

I know that the Gene Expression kits can capture 500-10,000 cells, but when you just need 500-5,000 cells/...
Forum: Sample Prep / Library Generation 03-14-2019, 12:59 PM
Replies: 10
Views: 1,766
Posted By seqsuave
Why are you working at 37C? Most TS I have seen...

Why are you working at 37C? Most TS I have seen is around 50C, this might help to prevent any mismatching of your TSO or RT primers.
Forum: Sample Prep / Library Generation 03-14-2019, 12:50 PM
Replies: 1
Views: 948
Posted By seqsuave
Most Versatile DNA Prep Kit

Hello Everyone!

I am interested in peoples' opinion on what they would consider to be the most versatile DNA prep kit/enzymes. Most DNA library prep methods are pretty consistent in terms of...
Forum: Sample Prep / Library Generation 03-14-2019, 12:35 PM
Replies: 1
Views: 1,993
Posted By seqsuave
AmpliSeq vs TruSeq

Now that Illumina sells AmpliSeq, I am trying understanding the difference between AmpliSeq for Illumina and TruSeq Targeted panels. From what I can tell AmpliSeq can probe for more targets, and deal...
Forum: Ion Torrent 05-09-2018, 01:46 PM
Replies: 8
Views: 7,465
Posted By seqsuave
I am looking for cheaper kits for the PGM for...

I am looking for cheaper kits for the PGM for around the same purpose, and while I have not done any testing yet, I found some kits that have barcodes included so you done have to worry about Ion...
Forum: Ion Torrent 05-01-2018, 01:33 PM
Replies: 9
Views: 8,739
Posted By seqsuave
Thats interesting and really cool, thanks for...

Thats interesting and really cool, thanks for that.
I didn't know the upgraded the S5, I wonder how the list prices compare to the S5 or S5 XL.

I have used S5 before, its a nice system I like it...
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