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Forum: Sample Prep / Library Generation 10-24-2016, 07:53 AM
Replies: 4
Views: 2,966
Posted By paramita7
atacseq from frozen tissue

Hello klebsiella, I am going to try the ENCODE protocol within few days. will update then. Mean time if you did try it already let me know how it went. Thanks,
Forum: Sample Prep / Library Generation 05-17-2016, 07:20 AM
Replies: 69
Views: 45,039
Posted By paramita7
Exclamation Hi Runuply, I have frozen brain tissue and I am...

Hi Runuply,
I have frozen brain tissue and I am just using grind and strain method. But after purification steps I am loosing a lot of DNA.
Forum: Sample Prep / Library Generation 05-05-2016, 06:07 AM
Replies: 69
Views: 45,039
Posted By paramita7
Question How did you dissociate cells from frozen tissue?

Hi Runuply,
i am also doing ATAC-seq from froze tissue. Can you give me an idea how you dissociate cells from tissue? I am having difficulty with it. Thank you,
Forum: Sample Prep / Library Generation 04-04-2016, 12:39 PM
Replies: 12
Views: 7,280
Posted By paramita7
sigma has .2-1uM range for adapter sequences

In atac-seq protocol they say 25uM of primer but sigma has .2-1um range. Can you tell how you ordered.Thanks,
Forum: Sample Prep / Library Generation 03-29-2016, 07:01 AM
Replies: 4
Views: 2,966
Posted By paramita7
Question ATAC-Seq cell preparation from Tissue

Hi,
I am a newbie in the field of ATAC-Seq. I am trying to start the protocol but my samples are Fresh/Frozen Tissue, not cell. I am having trouble deciding on how to dissociate cells from the...
Forum: Epigenetics 03-07-2016, 12:10 PM
Replies: 1
Views: 1,457
Posted By paramita7
ATAC-Seq method starting with extracted DNA instead of cells

I am new to try ATAC-seq method in my lab. The protocol from stanford has mentioned to start with 50,000 cells. I have already extracted DNA in my hand. is it possibly to use the gDNA for transpose...
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