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Forum: Illumina/Solexa 08-04-2017, 09:52 AM
Replies: 17
Views: 3,539
Posted By ScienceGrrl
Hello Arthur45, Make sure you have a very...

Hello Arthur45,

Make sure you have a very accurate quant of your input samples. Nextera is a mass driven setup so if you are off on your initial quant at all, can get results of too large/too...
Forum: General 07-06-2017, 02:22 PM
Replies: 1
Views: 1,716
Posted By ScienceGrrl
Hello SymphonicBio, If you still have any...

Hello SymphonicBio,

If you still have any programming questions for your STAR, please let me know. I can get you in contact with one of our specialists.

-Mary
Forum: Sample Prep / Library Generation 06-28-2016, 09:37 AM
Replies: 2
Views: 1,949
Posted By ScienceGrrl
For all SPRI products (AMPureXP, SPRIselect, etc)...

For all SPRI products (AMPureXP, SPRIselect, etc) if you go lower than 68% EtOH, you will cause the nucleic acid to elute off the beads. For manual prep, there is usually not an issue so earlier...
Forum: Illumina/Solexa 05-27-2016, 09:36 AM
Replies: 16
Views: 4,105
Posted By ScienceGrrl
Hello, I am the Scientific Leader, NGS for...

Hello,

I am the Scientific Leader, NGS for Hamilton Robotics. If you would like to speak with me before jumping into getting sales reps involved, feel free to contact me here or I can give you my...
Forum: Core Facilities 10-20-2015, 07:05 AM
Replies: 4
Views: 3,707
Posted By ScienceGrrl
I work for Hamilton Robotics as the Scientific...

I work for Hamilton Robotics as the Scientific Leader for NGS, but came from Beckman Coulter where I was the application scientist developing the NGS methods for them. If you would like to PM me and...
Forum: Sample Prep / Library Generation 09-16-2015, 09:59 AM
Replies: 5
Views: 1,491
Posted By ScienceGrrl
I had the opposite problem in my previous...

I had the opposite problem in my previous position of too much sample at the end b/c of failure to remove rRNA. I tested my depletion using Illumina's suggestion of running qPCR assay using GAPDH...
Forum: General 07-17-2015, 12:31 PM
Replies: 2
Views: 960
Posted By ScienceGrrl
Beckman has a protocol for miRNA selection using...

Beckman has a protocol for miRNA selection using their SPRIselect beads and isopropanol.
Forum: Sample Prep / Library Generation 07-17-2015, 09:53 AM
Replies: 6
Views: 2,652
Posted By ScienceGrrl
In my previous lab, we found that with certain...

In my previous lab, we found that with certain plastics, the adaptors were binding to it. When doing the dilution, we were using nuclease-free water sometimes and not the suggested 10 mM Tris-HCl...
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