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Forum: Ion Torrent 09-23-2012, 07:22 AM
Replies: 70
Views: 24,813
Posted By low99
How does the Proton produce 60-80 million reads?...

How does the Proton produce 60-80 million reads? My numbers don't add up.
The way I calculate reads is total output divided by bp.
So 10Gb/200bp = 50 million
Where are they getting the extra 30...
Forum: Introductions 08-29-2012, 09:05 AM
Replies: 2
Views: 1,193
Posted By low99
Welcome to Seqanswers! When are you getting your...

Welcome to Seqanswers! When are you getting your Proton? Would love to hear about your experience with this new instrument.
Forum: Ion Torrent 04-23-2012, 06:46 PM
Replies: 122
Views: 121,606
Posted By low99
I have to agree with ECO, for ease of use and...

I have to agree with ECO, for ease of use and data quality, the MiSeq is the best choice by far.
Nick Loman's comparison paper also highlights the systemic homopolymer issue that's inherent to the...
Forum: Ion Torrent 03-28-2012, 06:52 AM
Replies: 7
Views: 11,490
Posted By low99
Basically it will come down to three things:...

Basically it will come down to three things: output of your instrument, size of the genome and coverage required (what depth of sequencing do you need?)

314 chip highest output ~20Mb...
Forum: Ion Torrent 03-28-2012, 12:31 AM
Replies: 7
Views: 11,490
Posted By low99
It really depends on the coverage depth that you...

It really depends on the coverage depth that you need.
What coverage are you looking for?
Forum: Ion Torrent 03-04-2012, 09:40 PM
Replies: 15
Views: 15,454
Posted By low99
Unfortunately this is an inherent problem of the...

Unfortunately this is an inherent problem of the 454, Ion Torrent and probably the Proton chemistry. It is well documented. This if from the NEJM article on the sequencing on the German EColi...
Forum: Ion Torrent 02-19-2012, 08:39 PM
Replies: 88
Views: 156,967
Posted By low99
Actually the price on an ION is more like $250K...

Actually the price on an ION is more like $250K by the time you add the One Touch and server that you need for each ION unit....LT tends to only talk about the price of the sequencer and never...
Forum: Illumina/Solexa 02-16-2012, 06:15 PM
Replies: 4
Views: 2,972
Posted By low99
Screen shot try 2

Here it is. Let see if it works this time
Forum: Illumina/Solexa 02-16-2012, 05:39 PM
Replies: 4
Views: 2,972
Posted By low99
basespace blog screen shot

Here is a screen shot of the blog. Might have to register in the illumina site but is free to access basespace
Forum: Illumina/Solexa 02-16-2012, 02:12 PM
Replies: 4
Views: 2,972
Posted By low99
Illumina Genome in a day on HiSeq2500

Illumina 2500 completes 40x genome in 27hr! WOW..for someone like me who worked on the original Human Genome project this is amazing....

Can browse the data on Basespace...
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