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Forum: Sample Prep / Library Generation 08-20-2017, 04:58 PM
Replies: 7
Views: 3,310
Posted By RNA_
If contaminants are giving rise to the shoulder...

If contaminants are giving rise to the shoulder you might see evidence of them by nanodrop. Precipitating the RNA and washing 2 - 3x with 75% ethanol usually helps me.
Forum: Sample Prep / Library Generation 07-15-2017, 10:20 AM
Replies: 12
Views: 2,706
Posted By RNA_
I have this issue with Trizol sometimes. Two...

I have this issue with Trizol sometimes. Two or three washes with 75% ethanol, rather than the 1 wash in the protocol, usually prevents it from happening. I also never to try to recover all of...
Forum: Bioinformatics 07-15-2017, 09:58 AM
Replies: 1
Views: 823
Posted By RNA_
The Dali server searches for homology in solved...

The Dali server searches for homology in solved 3D structures.
I think a BLAST search should be good enough for your needs.
Forum: Sample Prep / Library Generation 02-26-2016, 11:16 PM
Replies: 3
Views: 1,688
Posted By RNA_
If your lab is licensed for radioactive work,...

If your lab is licensed for radioactive work, prepare a test library in parallel using trace amounts of 32P-labeled substrate spiked into your sample --> run on PAGE with appropriate radiolabeled...
Forum: Sample Prep / Library Generation 02-08-2016, 07:03 PM
Replies: 1
Views: 1,563
Posted By RNA_
I and my coworkers have had success diluting...

I and my coworkers have had success diluting lysates (in phosphate buffered saline or HEPES pH 7.5 + 100 mM NaCl) in RLT and recovering high quality large RNAs ( ~ >200 nt). Yield was high as we...
Forum: RNA Sequencing 01-24-2016, 04:13 PM
Replies: 4
Views: 1,937
Posted By RNA_
For anyone else interested: tDRMapper:...

For anyone else interested:

tDRMapper: http://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-015-0800-0
Forum: RNA Sequencing 11-23-2015, 09:45 AM
Replies: 4
Views: 1,937
Posted By RNA_
Thanks for the quick reply GenoMax. That's...

Thanks for the quick reply GenoMax. That's definitely helpful.
Forum: RNA Sequencing 11-23-2015, 09:34 AM
Replies: 4
Views: 1,937
Posted By RNA_
Hi Diego, I'm having the same problem now,...

Hi Diego,

I'm having the same problem now, but need to quantify tRNA fragments for a different purpose. I was thinking of making a tRNA "genome" based on the consensus sequences of each tRNA...
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