Forum: Ion Torrent
01-06-2014, 12:46 AM
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Replies: 5
Views: 4,086
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Forum: Ion Torrent
01-03-2014, 03:15 AM
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Replies: 5
Views: 4,086
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Forum: Bioinformatics
10-28-2013, 01:56 AM
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Replies: 0
Views: 1,498
make: *** [htscmd] Error 1
Hello All, I'm trying to build GATK on my iMac (OS X 10.9 Mavericks) but when installing htmlib I get the following;
vcfcheck.c:703:39: warning: format specifies type 'long' but the argument has...
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Forum: Ion Torrent
09-13-2013, 08:17 AM
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Replies: 8
Views: 11,169
In case anyone else is interested I downloaded...
In case anyone else is interested I downloaded some exome data from Ion Community and converted thee BAM files to put them into fastQC, the results are very similar to what we are seeing with Q20 to...
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Forum: Ion Torrent
09-10-2013, 01:41 AM
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Replies: 8
Views: 11,169
Ion Proton Q20
Hello All, we've only just done our first Proton run and the per base quality of the FastQC has ~90% of our data in the Q20 to Q28 range, should I be able to get up into the 30s or is it normal to be...
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Forum: SOLiD
09-04-2013, 08:43 AM
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Replies: 1
Views: 2,666
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Forum: Illumina/Solexa
09-04-2013, 08:40 AM
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Replies: 8
Views: 7,445
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Forum: Ion Torrent
07-30-2013, 03:03 AM
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Replies: 2
Views: 4,844
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Forum: SOLiD
07-10-2013, 04:06 AM
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Replies: 5
Views: 8,298
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Forum: SOLiD
06-10-2013, 08:07 AM
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Replies: 1
Views: 3,962
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Forum: Illumina/Solexa
05-24-2013, 12:18 AM
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Replies: 8
Views: 3,148
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Forum: Illumina/Solexa
05-23-2013, 07:51 AM
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Replies: 8
Views: 3,148
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Forum: Illumina/Solexa
05-16-2013, 07:50 AM
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Replies: 7
Views: 3,509
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Forum: General
05-15-2013, 08:37 AM
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Replies: 26
Views: 5,132
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Forum: Illumina/Solexa
05-15-2013, 08:03 AM
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Replies: 7
Views: 3,509
Hi microgirl, we are pooling the PCR then...
Hi microgirl, we are pooling the PCR then prepping it as one library so there are no adapters to check for at the pooling stage. When we pool 2 libraries we get good balance between the samples but...
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Forum: General
05-15-2013, 07:54 AM
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Replies: 26
Views: 5,132
In the most simple terms you are saying "if you...
In the most simple terms you are saying "if you can detect the cells and kill them you would have a cure" then 'Yes' that is true but that does not take into account the complexity of the biological...
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Forum: General
05-15-2013, 06:25 AM
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Replies: 26
Views: 5,132
It's hard to explain why the answer to this is...
It's hard to explain why the answer to this is 'No' as I'm guessing you don't have much of a background in genetics so I'll try a computer based analogy.
Your anti virus software can identify and...
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Forum: Illumina/Solexa
05-15-2013, 12:35 AM
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Replies: 7
Views: 3,509
We shear with the Covaris and check it with the...
We shear with the Covaris and check it with the Bioanalyser, we've also checked with agarose gel to see if there is a significant quantity of un-sheared product, on top of that we've seen the same...
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Forum: Illumina/Solexa
05-14-2013, 08:40 AM
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Replies: 7
Views: 3,509
pooling long range PCR
Hello, I have an experiment with 20 over lapping long range PCR fragments that span a single gene. The average size is 5kb (max 10, min 1.5kb) with equimolar pooling the balance between fragments is...
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Forum: SOLiD
05-01-2013, 04:38 AM
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Replies: 25
Views: 11,764
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Forum: SOLiD
05-01-2013, 03:08 AM
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Replies: 2
Views: 1,704
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Forum: SOLiD
05-01-2013, 03:05 AM
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Replies: 2
Views: 1,833
Hi jayalakshmi
we've seen this problem...
Hi jayalakshmi
we've seen this problem before and traced the issue back to a problem with quantification, what method do you use?
Too much input DNA leads to polyclonal beads on the slide which...
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Forum: SOLiD
04-29-2013, 04:32 AM
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Replies: 6
Views: 2,490
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Forum: SOLiD
04-29-2013, 04:28 AM
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Replies: 25
Views: 11,764
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Forum: SOLiD
04-26-2013, 08:23 AM
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Replies: 3
Views: 5,004
With Solid data you need to do the mapping in...
With Solid data you need to do the mapping in base-space, not convert the fastq and map that. If you're not familiar with it I would recommend tracking down an expert.
I don't know if it is still...
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