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Forum: Oxford Nanopore 12-18-2018, 12:23 PM
Replies: 2
Views: 2,281
Posted By aharring
So ONT contacted me with an answer to my...

So ONT contacted me with an answer to my question.
The Blunt/TA ligase master mix was validated in-house for the ligation of the barcodes. However, feel free to just use T4 from the Quick Ligation...
Forum: Oxford Nanopore 12-17-2018, 08:42 PM
Replies: 2
Views: 2,281
Posted By aharring
Few questions about minion

I want to obtain long read data for 6 bacterial isolates. I have purchased the minion starter kit with the ligation kit and native barcode kit. I am confused on why the protocol requires the...
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