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Forum: Bioinformatics 08-09-2008, 10:35 AM
Replies: 2
Views: 3,473
Posted By Macki1x
maq on a mac

Hello,

I have downloaded my first solexa 1g sage data set in fastq format from a collaborator with a 1G machine (1.9GB) and wish to analyze it.

They recommended maq, and I downloaded maq from...
Forum: Bioinformatics 07-30-2008, 01:33 PM
Replies: 1
Views: 5,602
Posted By Macki1x
Reference Genome

Hello,

I have data in FASTQ format from the Illumina 1G Analyzer. I wish to analyze it with Maq. The library represents gene expression TAGs derived from mouse mRNA.

I need to find a mouse...
Forum: Illumina/Solexa 05-09-2008, 02:11 PM
Replies: 6
Views: 7,980
Posted By Macki1x
solexa seq

I spent alot of time dealing with an adaptor-adaptor concatamer that formed during the reaction and swamped-out and/or killed the formation of the desired Ad1-Tag-Ad2 product.

I realize now that...
Forum: Illumina/Solexa 12-18-2007, 10:25 AM
Replies: 6
Views: 7,980
Posted By Macki1x
Thanks for your suggestion regarding the gel...

Thanks for your suggestion regarding the gel purification step performed prior to pcr amplification.

So far, I have been following the Solexa protocol that accompanies their kit (Solexa Gene...
Forum: Illumina/Solexa 12-18-2007, 08:29 AM
Replies: 6
Views: 7,980
Posted By Macki1x
Solexa Protocol

Has anyone had success generating Adaptor1-TAG-Adaptor2 products for Solexa Sequencing?

I am trying to get this working and am only able to make Adaptor 2 concatamers. Basically, I extract RNA...
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