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Forum: Bioinformatics 03-23-2017, 11:27 AM
Replies: 2
Views: 3,622
Posted By alexbenroland
Hi Nandan, I hope you fixed your problem...

Hi Nandan,

I hope you fixed your problem since that time.
Anyway, I think you need to convert your file in the PGDSpider own format (PGD), then concert to Bayescan format. I was having the same...
Forum: General 03-03-2015, 01:48 PM
Replies: 6
Views: 3,156
Posted By alexbenroland
I was wondering what could be the best choice in...

I was wondering what could be the best choice in RE combination+size selection, to obtain a good sampling of the genome.
Let's say for the same number of loci (10,000 as an example), I have the...
Forum: General 03-02-2015, 12:56 PM
Replies: 6
Views: 3,156
Posted By alexbenroland
We have access to a Pippin prep, so I will use...

We have access to a Pippin prep, so I will use that for size selection



I agree, designing this experiment start to be tricky.
I wonder if it's might not be interesting to make it in 2 times....
Forum: General 03-01-2015, 10:45 AM
Replies: 6
Views: 3,156
Posted By alexbenroland
Hi SNPsaurus, Thanks for your fast answer. ...

Hi SNPsaurus,
Thanks for your fast answer.
Here are some precision about the project:



We have around 200 samples from 14-15 populations plus extra closely related sisters species that we...
Forum: General 02-27-2015, 07:07 AM
Replies: 6
Views: 3,156
Posted By alexbenroland
questions for ddRAD seq project on frogs

Hi everyone,

I'm working on a population genetic project involving a non-model organism of frog.
We decided to use the ddRADseq to ask some population genetic questions.
I started to dig into...
Forum: Introductions 02-27-2015, 06:19 AM
Replies: 0
Views: 931
Posted By alexbenroland
Hello all

Hi everybody,
I'm a postdoc in Boston area, working with frogs and using Next-gen sequencing tools (RNA-seq and starting ddRAD-seq).
I have a small experience with RNA-seq and none for ddRAD-seq,...
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