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Forum: Illumina/Solexa 05-01-2014, 11:02 AM
Replies: 0
Views: 1,116
Posted By ASL
Question Illumina TruSeq adaptor issue?

Hello everyone,

I am using the Illumina TruSeq ChIP Sample Prep kit (Set A adaptors) to generate my ChIP-Seq libraries. One of the indexes (#19) gives me libraries that look okay on the...
Forum: Bioinformatics 10-26-2013, 02:02 PM
Replies: 10
Views: 9,728
Posted By ASL
Unhappy Easy way to sftp file for Galaxy upload?

Does anyone know of an easy way to upload fastq files via sftp to the main Galaxy at Penn State or to a local instance of Galaxy?

We are in the process of setting up a local instance of Galaxy...
Forum: Sample Prep / Library Generation 04-11-2013, 01:14 PM
Replies: 0
Views: 1,704
Posted By ASL
Unhappy Do nuclei need to be isolated at beginning of prep?

I am using a ChIP protocol based on that described in Zhang L et al (2009) Amer J Nephrol 30(6):505-513, for PBMCs. The cells are resuspended in buffer and lysed by sonication, I have seen other...
Forum: Sample Prep / Library Generation 04-09-2013, 11:59 AM
Replies: 1
Views: 5,931
Posted By ASL
NEBNext ChIP-Seq library prep kit vs Illumina TruSeq kit?

Has anyone used the NEBNext ChIP-Seq Library Prep Master Mix Set (or Reagent Set) for Illumina and gotten good results?

I bought this kit on someone else's recommendation, and I'm having my...
Forum: Introductions 04-09-2013, 11:43 AM
Replies: 0
Views: 844
Posted By ASL

Hi, I just joined! I'm working on a new ChIP-seq project which is new to me so I was happy to discover this bulletin board! :)
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