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Forum: Sample Prep / Library Generation 08-07-2017, 08:44 AM
Replies: 2
Views: 1,348
Posted By candida utilis
DNase treatment for SMART Seq2

I notice that DNase I treatment is not included in the SMART Seq2 protocol. May I know how efficient Oligo-dT30VN will bind to mRNA only? Is there any possibility that Oligo-dT30VN can also amplify...
Forum: Sample Prep / Library Generation 06-10-2017, 08:20 PM
Replies: 6
Views: 2,184
Posted By candida utilis
Hi Minukk, thanks for your reply. We didn't use...

Hi Minukk, thanks for your reply. We didn't use Minukk's strategy because the RNA amount between cell types is quiet different. But we can think about this strategy.

Hi Simone78, thanks for your...
Forum: Sample Prep / Library Generation 06-02-2017, 09:35 AM
Replies: 6
Views: 2,184
Posted By candida utilis
Hi Simone, thanks for reply. But I think my words...

Hi Simone, thanks for reply. But I think my words mislead you. I didn't use all RNA for both samples. Instead, for 50,000 cells, I only use 3ng RNA with 15 pre-amplification cycles, no matter how...
Forum: Sample Prep / Library Generation 05-08-2017, 12:18 AM
Replies: 6
Views: 2,184
Posted By candida utilis
SMART Seq v2: RNA amount and cycles for preampfification

Hi all,
We aim to compare differential mRNA expression between 2 different cell types. We sorted 2 populations of cells by FACS, extract RNA by column based method, and SMARTSEQ v2 for...
Forum: Sample Prep / Library Generation 05-07-2017, 11:56 PM
Replies: 0
Views: 731
Posted By candida utilis
SMART Seq v2: RNA amount and cycles for preampfification

Hi all,
We aim to compare differential mRNA expression between 2 different cell types. We sorted 2 populations of cells by FACS, extract RNA by column based methods, and SMARTSEQ v2 for...
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