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Forum: Illumina/Solexa 05-09-2016, 07:33 AM
Replies: 8
Views: 1,221
Posted By wendy_H
Cool! I will look into that. Thank you!

Cool! I will look into that. Thank you!
Forum: Illumina/Solexa 05-09-2016, 07:24 AM
Replies: 8
Views: 1,221
Posted By wendy_H
Yeah, so we don't have instrument for beads...

Yeah, so we don't have instrument for beads purification either... and we don't have a sequencing facility, so I did everything by myself in-house.

I wish I could pay sequencing company/facility...
Forum: Illumina/Solexa 05-09-2016, 07:09 AM
Replies: 8
Views: 1,221
Posted By wendy_H
To GenoMax, Unfortunately we don't have BA...

To GenoMax,

Unfortunately we don't have BA and can't afford to have it for now :(

To remove the smaller inserts, I did gel extraction (to remove fragment length less than 500bp), but it didn't...
Forum: Illumina/Solexa 05-07-2016, 06:18 AM
Replies: 8
Views: 1,221
Posted By wendy_H
It's number 1. The most recent MiSeq was run...

It's number 1. The most recent MiSeq was run using 2x300bp, in about 20% of the sequencing output, I get about 60bp read length followed by low quality nucleotides, which I think indicate number 1...
Forum: Illumina/Solexa 05-06-2016, 07:37 PM
Replies: 8
Views: 1,221
Posted By wendy_H
20% adapter get sequenced using MiSeq

Hi all,

I am new to MiSeq sequencing and it really frustrates me lately.

I had two MiSeq run recently and they all turned out to have about 20% adapter get sequenced. I've been using the TruSeq...
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