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Forum: Illumina/Solexa 02-27-2020, 02:15 AM
Replies: 4
Views: 1,119
Posted By Dornfield
287 views and no responses! Does that mean no...

287 views and no responses! Does that mean no one knows, or no one is interested? It is just a academic question really: how small can Illumina go with this?
Forum: Illumina/Solexa 02-20-2020, 02:40 AM
Replies: 4
Views: 1,119
Posted By Dornfield
NextSeq 1000 and 2000 cluster density and number of copies

Any knowledge on the cluster dimensions of the new systems, how many copies of the library are present in each cluster and how these flow cells differ from the other patterned arrays that have become...
Forum: Ion Torrent 04-10-2018, 07:53 AM
Replies: 1
Views: 2,946
Posted By Dornfield
Droplets, beads & wells

Hi,

Can anyone point me in the direction of a post or publication that lists how the droplet size for emPCR, the bead size and the well dimensions of the various Ion chips vary? Are all of the...
Forum: Illumina/Solexa 10-03-2017, 06:30 AM
Replies: 4
Views: 1,337
Posted By Dornfield
Thanks. I had seen that patent before, but not...

Thanks. I had seen that patent before, but not thought to do some very back of the evenlope calculations:

250nm diameter features means a surface ares of ~49,000nm^2. My rule of thumb for a...
Forum: Illumina/Solexa 10-03-2017, 05:48 AM
Replies: 4
Views: 1,337
Posted By Dornfield
Perhaps I could come at this from a different...

Perhaps I could come at this from a different direction (or invite educated guesses?). I am not actually an Illumina user, so I'm not familiar with the workflow that generates the clusters in the...
Forum: Sample Prep / Library Generation 10-02-2017, 01:14 AM
Replies: 1
Views: 949
Posted By Dornfield
This thread now more appropriately reposted, by...

This thread now more appropriately reposted, by the author, in the Illumina/ Solexa forum.
Forum: SOLiD 10-02-2017, 01:00 AM
Replies: 3
Views: 7,398
Posted By Dornfield
I realise that this is replying to a very old...

I realise that this is replying to a very old Thread, but my understanding is that Wildfire was the clonal amplification technology applied to SOLiD, whereas Avalanche was the (same) technology...
Forum: Illumina/Solexa 10-02-2017, 12:49 AM
Replies: 4
Views: 1,337
Posted By Dornfield
Clonal amplification/ Kinetic Exclusion parameters

Reposted from Library Genertation Forum, where I somehow managed to mispost it...

Newer generation Illumina platforms with patterened flowcells use kinetic exclusion to achieve a high degree of...
Forum: Sample Prep / Library Generation 10-02-2017, 12:39 AM
Replies: 1
Views: 949
Posted By Dornfield
Clonal Amplification/ Kinetic Exclusion

Newer generation Illumina platforms with patterened flowcells use kinetic exclusion to achieve a high degree of clonal chastity. From what I understand, the amplification method is still bridge, but...
Forum: Bioinformatics 09-20-2016, 01:39 AM
Replies: 3
Views: 2,381
Posted By Dornfield
Physical removal of adapter/ primer sequences

Slightly off topic, but definitely related, I've been doing a little hunting on the physical removal of the remnants of adapters or, more likely, the primer remnants of a library generated by...
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