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Forum: General 01-16-2017, 09:39 AM
Replies: 7
Views: 1,908
Posted By AllSeq
Adding "time to sequence" is an interesting idea,...

Adding "time to sequence" is an interesting idea, but it seems complicated. I was keeping the list at a pretty high level - e.g., Illumina vs PacBio. The time to sequence would vary depending on a...
Forum: General 01-16-2017, 09:35 AM
Replies: 7
Views: 1,908
Posted By AllSeq
I specifically avoided Sanger sequencing, partly...

I specifically avoided Sanger sequencing, partly out of laziness and partly because people usually refer to 'generations' in 'next generation' sequencing. That said, I would agree with your...
Forum: General 01-14-2017, 04:28 PM
Replies: 7
Views: 1,908
Posted By AllSeq
That's a really good point!

That's a really good point!
Forum: General 01-14-2017, 11:20 AM
Replies: 7
Views: 1,908
Posted By AllSeq
The Futility of Defining Sequencing "Generations"

I just recently answered a question on Quora about the number of “sequencing generations” there are. (My answer was basically that the concept of generations for sequencing has never been used...
Forum: Illumina/Solexa 01-14-2017, 08:44 AM
Replies: 112
Views: 17,519
Posted By AllSeq
I'm pretty sure they meant 80% of the running...

I'm pretty sure they meant 80% of the running cost (per Gb), not 80% of the specific kit cost. However, we've still only seen hints at specific pricing, so we can't say for sure.
Forum: Illumina/Solexa 01-10-2017, 11:52 AM
Replies: 112
Views: 17,519
Posted By AllSeq
@illumina also just confirmed 2-color via Twitter.

@illumina also just confirmed 2-color via Twitter.
Forum: Oxford Nanopore 01-06-2017, 04:56 PM
Replies: 11
Views: 2,399
Posted By AllSeq
Cool. I've been out of the lab for a while and...

Cool. I've been out of the lab for a while and just wanted to make sure I hadn't completely lost it ;-)
Forum: Oxford Nanopore 01-06-2017, 04:44 PM
Replies: 11
Views: 2,399
Posted By AllSeq
Conceptually I agree with your explanation, but...

Conceptually I agree with your explanation, but surely 200ng doesn't represent 10^12 human genomes. One human genome is ~6 pg. 200ng = 200,000pg, so it's roughly 33k genomes-worth of DNA, right?
Forum: General 12-15-2016, 12:15 PM
Replies: 1
Views: 1,275
Posted By AllSeq
We can't comment specifically on these...

We can't comment specifically on these bioinformatics providers, but feel free to check out our extensive list of providers on AllSeq's Sequencing Marketplace (http://ngs.is/AllSeqProviders). You can...
Forum: Pacific Biosciences 12-15-2016, 12:05 PM
Replies: 62
Views: 16,590
Posted By AllSeq
For those who may have missed it - Roche...

For those who may have missed it - Roche Diagnostics pulled out of their marketing agreement with PacBio:...
Forum: General 12-04-2016, 11:03 PM
Replies: 3
Views: 1,560
Posted By AllSeq
The company using Helicos technology is SeqLL....

The company using Helicos technology is SeqLL. You can mark all of the 454 platforms as discontinued - they're all gone and no longer being supported. I would remove 10X Genomics as it isn't really a...
Forum: Vendor Forum 11-17-2016, 01:41 PM
Replies: 105
Views: 39,815
Posted By AllSeq
The NextSeq uses 2 color chemistry, but the...

The NextSeq uses 2 color chemistry, but the 3000/4000 uses the 'standard' 4 color chemistry (with patterned flow cells, just like the X).
Forum: Vendor Forum 11-17-2016, 10:56 AM
Replies: 105
Views: 39,815
Posted By AllSeq
Why would the NextSeq and HiSeq 3000/4000 be...

Why would the NextSeq and HiSeq 3000/4000 be similar? The use different chemistries and different flow cells. Wouldn't the 3000/4000 be most similar to the HiSeq X? (Or did you just mean they're...
Forum: Illumina/Solexa 10-31-2016, 10:07 AM
Replies: 2
Views: 530
Posted By AllSeq
The IGN was rolled out specifically for human...

The IGN was rolled out specifically for human whole genome sequencing and was (is?) probably being used to help keep the surprisingly large number of HiSeq X machines out there as busy as possible....
Forum: Core Facilities 09-03-2016, 12:11 PM
Replies: 7
Views: 1,555
Posted By AllSeq
For any core labs looking to increase their user...

For any core labs looking to increase their user base, be sure that you're listed on AllSeq's Sequencing Marketplace (http://allseq.com). It's a free way to advertise your core lab, both inside and...
Forum: Service Providers 06-22-2016, 10:21 AM
Replies: 162
Views: 119,224
Posted By AllSeq
If you're looking for a sequencing service...

If you're looking for a sequencing service provider, be sure to check out our list at AllSeq - http://allseq.com/providers/

The AllSeq Sequencing Marketplace is completely free - it's free for...
Forum: Pacific Biosciences 06-17-2016, 07:44 PM
Replies: 62
Views: 16,590
Posted By AllSeq
We don't have any data, but we have a (growing)...

We don't have any data, but we have a (growing) list of service providers who have installed the Sequel platform: http://ngs.is/PBSequel
Forum: Epigenetics 06-02-2016, 10:45 AM
Replies: 9
Views: 1,960
Posted By AllSeq
We've found that RRBS is an application that a...

We've found that RRBS is an application that a lot of providers are simply not comfortable running. While it's not a direct answer to your question, I'd encourage you to try out AllSeq's Sequencing...
Forum: Pacific Biosciences 04-14-2016, 07:04 PM
Replies: 50
Views: 8,539
Posted By AllSeq
I'm not quite sure. We're on their distribution...

I'm not quite sure. We're on their distribution list because we periodically consult for them on the genomics market.
Forum: Pacific Biosciences 04-14-2016, 03:51 PM
Replies: 50
Views: 8,539
Posted By AllSeq
We still haven't seen any of the data ourselves,...

We still haven't seen any of the data ourselves, but William Blair (an equity research firm) has had some positive feedback from users. From their research report earlier today:

"Early feedback...
Forum: Pacific Biosciences 03-23-2016, 05:13 PM
Replies: 50
Views: 8,539
Posted By AllSeq
Keith wasn't trying to pull a fast one on you. He...

Keith wasn't trying to pull a fast one on you. He got a DMCA takedown notice for an image of the RSII (Why? No idea.) Rather than fight it, he just removed the image. Apparently he wasn't able to...
Forum: Pacific Biosciences 03-21-2016, 02:42 PM
Replies: 50
Views: 8,539
Posted By AllSeq
From our perspective, PacBio's advantages are...

From our perspective, PacBio's advantages are true long reads and low consensus error rates. Total output improvements (in terms of Gb and # of reads) hasn't really been their strength (although the...
Forum: Vendor Forum 03-21-2016, 09:17 AM
Replies: 1
Views: 1,302
Posted By AllSeq
We're happy to add you to our list! You can find...

We're happy to add you to our list! You can find your profile here: Quick Biology (http://allseq.com/providers-list/quick-biology/).

Someone from our accounts team will be in touch with you...
Forum: Oxford Nanopore 03-11-2016, 10:03 AM
Replies: 4
Views: 2,001
Posted By AllSeq
Matching the error rate of PacBio would indeed be...

Matching the error rate of PacBio would indeed be pretty impressive, but that alone is insufficient to kill off PacBio or Illumina. The question is does the ONT error profile match that of PacBio? In...
Forum: Oxford Nanopore 02-25-2016, 02:29 PM
Replies: 0
Views: 1,568
Posted By AllSeq
MinION service providers

With the rising popularity of Oxford Nanopore’s sequencing platform, we’ve started to see some service providers offer MinION sequencing. We’ve found five so far...
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