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Forum: Ion Torrent 10-12-2019, 05:40 AM
Replies: 1
Views: 161
Posted By ChiaraGen
Emulsion Pcr

Hello,

I have a curiosity:the emulsion Pcr in Ion Torrent or in 454 Roche takes place inside the instrument as the bridge pcr in Illumina platforms or it happens outside the machine and then I...
Forum: Illumina/Solexa 08-12-2019, 10:43 AM
Replies: 3
Views: 607
Posted By ChiaraGen
Thank you very much for your help! What about...

Thank you very much for your help!
What about Q2, I have found a paper
https://www.nature.com/articles/s41598-017-03448-8

where the author writes:

"DNA exists naturally as a double-stranded...
Forum: Illumina/Solexa 08-11-2019, 12:08 AM
Replies: 3
Views: 607
Posted By ChiaraGen
NGS sequencing and capture probes

Hello!

I have two questions for you:

1.I don't understand,when a library is denatured before loading in the ngs instrument, I'll have the two complementary strands separately; so they will form...
Forum: Illumina/Solexa 08-01-2019, 06:07 AM
Replies: 0
Views: 551
Posted By ChiaraGen
Karyomapping Illumina

Hello!

Someone Knows how does it works Karyomapping by Illumina? I know that by SNPs array analysis it permits to reconstruct haplotypes and to knows if a particular mutation is transmitted to...
Forum: Bioinformatics 07-31-2019, 12:05 AM
Replies: 0
Views: 439
Posted By ChiaraGen
Off-target

Hello,
I have some questions about off-target. If I have understand well off-target can rise from probes that have promiscuous hybridization and recognize more sites in the genome, but also it can...
Forum: Illumina/Solexa 07-11-2019, 04:36 PM
Replies: 0
Views: 721
Posted By ChiaraGen
Pcr bridge error rate and duplicates

Hello!
I have 3 questions,can you help me please to understand?
1. Why almost only the pcr step in the library prep is cited as source of errors while bridge amplification not?
I have found only...
Forum: Sample Prep / Library Generation 03-26-2019, 04:23 AM
Replies: 0
Views: 505
Posted By ChiaraGen
Haloplex HS method

Hello!
I have two questions about Haloplex HS protocol:

1. I read that Haloplex uses double stranded byotinilated probes and during hybridization step I put with probes also indexes. Someone...
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