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Search: Posts Made By: DNAcowboy
Forum: Bioinformatics 12-02-2012, 05:49 PM
Replies: 0
Views: 739
Posted By DNAcowboy
Processing Speed and Pipelines

Hello,

I'm trying to understand which of Illumina or Life has the fastest Bio-IT pipeline for the processing of data.

Once you get the raw data, let's say a 30X genome set, how long will it...
Forum: General 08-13-2009, 02:16 AM
Replies: 67
Views: 78,036
Posted By DNAcowboy
Nice Work James, congratulation. Could you...

Nice Work James, congratulation.

Could you let us know the grand total number of instrument you have collected until now?

I read recently this paper:...
Forum: 454 Pyrosequencing 07-28-2009, 06:41 AM
Replies: 1
Views: 1,909
Posted By DNAcowboy
I have visited a lab where all 3 instruments...

I have visited a lab where all 3 instruments share the same single large room. I guess the chemistry part was performed in various other rooms, just like any PCR setup. Illumina Cluster stations were...
Forum: Illumina/Solexa 02-08-2009, 02:15 PM
Replies: 74
Views: 363,032
Posted By DNAcowboy
Interesting. Thanxx.

Interesting. Thanxx.
Forum: Illumina/Solexa 02-08-2009, 01:59 PM
Replies: 74
Views: 363,032
Posted By DNAcowboy
Any information about the way Illumina will...

Any information about the way Illumina will achieve 250b reads? like they recently announced.
Forum: 454 Pyrosequencing 01-05-2009, 01:29 PM
Replies: 26
Views: 41,138
Posted By DNAcowboy
These documents are of a great help. Do they...

These documents are of a great help.
Do they exist for the other instruments?


C'mon Mr Illumina and Mr AB, we need some information like your friend's Mr Roche.
Forum: Events / Conferences 01-05-2009, 12:57 PM
Replies: 0
Views: 1,873
Posted By DNAcowboy
LabAutomation2009

Anyone going there?

I heard about nanopore sequencing for the 1st time at Labautomation, years ago.

I'll be there.

LabAutomation2009 (http://www.labautomation.org/la09/index.cfm)
Forum: General 12-03-2008, 12:54 PM
Replies: 5
Views: 2,484
Posted By DNAcowboy
thanxx for your comments Scott.

thanxx for your comments Scott.
Forum: General 12-03-2008, 12:07 AM
Replies: 5
Views: 2,484
Posted By DNAcowboy
Thanxx Scott, it helps. I guess Roche and...

Thanxx Scott, it helps. I guess Roche and LifeTech. instrument users will need more than that.

How much data storage space did you add yourself to feel comfortable?
BTW, could you tell us if...
Forum: Bioinformatics 12-02-2008, 04:34 AM
Replies: 7
Views: 3,265
Posted By DNAcowboy
Obviously they are LIMS for NextGen experiments....

Obviously they are LIMS for NextGen experiments. But considering the level of investment I rather like my future LIMS to handle more than just the nextgen experiments.

I wish I could find a lims...
Forum: General 12-02-2008, 01:56 AM
Replies: 5
Views: 2,484
Posted By DNAcowboy
Additional accessories for NGS

Hello,

Can we try to build a list of additionial pieces of equipment needed with the GFLX, GA and Solid?

I read about sonicators, gel control, mag. beads counter,...up to disk storage but this...
Forum: Bioinformatics 12-02-2008, 01:28 AM
Replies: 7
Views: 3,265
Posted By DNAcowboy
I shall appreciate too some comments on LIMS. ...

I shall appreciate too some comments on LIMS.
My understanding is that Geospiza or GenoLogic are not LIMS. They are additionnal pieces directly addressing NextGen data processing only.

They are...
Forum: SOLiD 05-29-2008, 01:33 AM
Replies: 67
Views: 163,003
Posted By DNAcowboy
This is my understanding, the 16 possible 2...

This is my understanding, the 16 possible 2 base-combinations for the oligos are present in the mix, not only those 4 on their diagrams. Am I wrong?
Forum: Illumina/Solexa 05-23-2008, 04:04 AM
Replies: 74
Views: 363,032
Posted By DNAcowboy
Recently upgraded on illumina's web site but not...

Recently upgraded on illumina's web site but not obvious to find:
http://www.illumina.com/seminars/archive/Illumina_GA_Update.pdf
Forum: Events / Conferences 04-25-2008, 04:36 AM
Replies: 21
Views: 8,965
Posted By DNAcowboy
Are these cost nbrs recent? Mine are extra fresh...

Are these cost nbrs recent? Mine are extra fresh from the day from AB Europe. Price list for reagents/cost excl. VAT

SOLID 4Gb
Slide 1 "section": €2600->2600/section
Slide 4: €3700->~900/section...
Forum: Events / Conferences 04-25-2008, 04:18 AM
Replies: 21
Views: 8,965
Posted By DNAcowboy
:eek:That's really, really an awesome link. Thanx...

:eek:That's really, really an awesome link. Thanx so much for sharing this info.
Forum: SOLiD 04-16-2008, 09:05 AM
Replies: 67
Views: 163,003
Posted By DNAcowboy
Thanxx Eco. I should be visited soon by AB's...

Thanxx Eco. I should be visited soon by AB's representatives and should post some answers asap.
Forum: SOLiD 04-16-2008, 07:42 AM
Replies: 67
Views: 163,003
Posted By DNAcowboy
I have difficulties finding the SOLID instrument...

I have difficulties finding the SOLID instrument specs.
How long will be a run? How much data will it generate? Do we have these published somewhere?
Forum: Events / Conferences 04-09-2008, 10:11 PM
Replies: 2
Views: 3,744
Posted By DNAcowboy
Seems to be a private Roche-454 user meeting for...

Seems to be a private Roche-454 user meeting for europeans, on invitation only. Info was obtained from Roche. Scientific program:

Sequence Capture Technology
Dr. David Wheeler

Gene-specific...
Forum: General 04-09-2008, 06:24 AM
Replies: 19
Views: 6,507
Posted By DNAcowboy
It sure does help, thanxx. People around me,...

It sure does help, thanxx.

People around me, for sure are working on a small number of genes but you'll find funny how this number exponentially increases when you mention the nextgen sequencers...
Forum: Events / Conferences 04-09-2008, 12:20 AM
Replies: 2
Views: 3,744
Posted By DNAcowboy
Forum: Events / Conferences 04-09-2008, 12:16 AM
Replies: 3
Views: 3,981
Posted By DNAcowboy
Live Blogging from the CHI Next Gen Conference! (San Diego, CA): Updated Day 1

So if you are there, can you plan to retrieve for us the slide presentations? That should be awesome.

PS: forget about camcording the talks ;-))
Forum: General 04-08-2008, 08:43 AM
Replies: 19
Views: 6,507
Posted By DNAcowboy
This means you cannot "re-use" a flow cell. that...

This means you cannot "re-use" a flow cell. that would have run just a few lanes?
Forum: General 04-08-2008, 07:00 AM
Replies: 19
Views: 6,507
Posted By DNAcowboy
I don't know yet how the GA works at the bench...

I don't know yet how the GA works at the bench side, so does this means you can run individual lanes or do you need to run the full 8 lanes in one shot?



Do you mean $2 per 7-800b read? for...
Forum: General 04-08-2008, 06:27 AM
Replies: 19
Views: 6,507
Posted By DNAcowboy
ok guys, i've modified helicos cost according a...

ok guys, i've modified helicos cost according a document called "Investor Presentation
JP Morgan 2008 Healthcare Conference
San Francisco, CA
January 10, 2008" that, if I remember well, I've...
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