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Forum: Bioinformatics 04-03-2015, 01:03 AM
Replies: 63
Views: 9,018
Posted By joshquick
When you decreased the SPRI ratio you increased...

When you decreased the SPRI ratio you increased the insert size and we found bowtie2 behaves weirdly above 500bp inserts with the default settings. You might want to change your bowtie2 settings or...
Forum: Illumina/Solexa 09-23-2012, 04:31 AM
Replies: 37
Views: 43,191
Posted By joshquick
Hi Tony, 1) Agreed it looks about 2x that....

Hi Tony,

1) Agreed it looks about 2x that. lower reported density than you see in the Thumbnails could be caused by registration failures, you should see 0's in the P90 A/C/G/T if there was a...
Forum: Illumina/Solexa 08-10-2012, 01:47 PM
Replies: 6
Views: 9,263
Posted By joshquick
Hi, Nick and I have just written a blog post...

Hi,

Nick and I have just written a blog post on sequencing low-diversity on the MiSeq, may be of interest to you...
...
Forum: General 08-10-2012, 01:21 PM
Replies: 1
Views: 2,160
Posted By joshquick
Hi, It's possible, the easiest way is to...

Hi,

It's possible, the easiest way is to prise the lids off the two cartridges and use the SBS reagents (3 larger pots) from one to top up the other. You can peel back the foil and fill them up...
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