Forum: Bioinformatics
04-03-2015, 01:03 AM
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Replies: 63
Views: 9,018
When you decreased the SPRI ratio you increased...
When you decreased the SPRI ratio you increased the insert size and we found bowtie2 behaves weirdly above 500bp inserts with the default settings. You might want to change your bowtie2 settings or...
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Forum: Illumina/Solexa
09-23-2012, 04:31 AM
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Replies: 37
Views: 43,191
Hi Tony,
1) Agreed it looks about 2x that....
Hi Tony,
1) Agreed it looks about 2x that. lower reported density than you see in the Thumbnails could be caused by registration failures, you should see 0's in the P90 A/C/G/T if there was a...
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Forum: Illumina/Solexa
08-10-2012, 01:47 PM
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Replies: 6
Views: 9,263
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Forum: General
08-10-2012, 01:21 PM
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Replies: 1
Views: 2,160
Hi,
It's possible, the easiest way is to...
Hi,
It's possible, the easiest way is to prise the lids off the two cartridges and use the SBS reagents (3 larger pots) from one to top up the other. You can peel back the foil and fill them up...
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