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Forum: Sample Prep / Library Generation 01-30-2019, 03:27 PM
Replies: 16
Views: 4,355
Posted By evan_seq24
It was recommended that we use Ribominus or...

It was recommended that we use Ribominus or Microbexpress as an alternative, but I am having no success with either kit. I sequenced some samples that passed Bioanalyzer qc (no visible residual rRNA)...
Forum: Sample Prep / Library Generation 09-21-2016, 01:28 PM
Replies: 3
Views: 1,636
Posted By evan_seq24
Thank you both for the thoughtful responses....

Thank you both for the thoughtful responses. Looking at my data, I do see a direct correlation between fragment size and clustering efficiency. I appreciate it!
Forum: Sample Prep / Library Generation 09-19-2016, 11:28 AM
Replies: 3
Views: 1,636
Posted By evan_seq24
Indexing QC distribution

Hi all,

First thread here! My question involves optimizing indexing QC using the Nextera XT library prep kit for DNAseq via Miseq. I'm wondering if anyone can suggest a method for tightening up...
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