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Forum: Illumina/Solexa 02-21-2017, 01:00 PM
Replies: 8
Views: 2,876
Posted By tacana
Hello all, I was wondering what was the...

Hello all,

I was wondering what was the resolution of rogerzzw problem? We are having similar issues with our next seq. and would love to hear how was this resolved.

Thanks in advance,

Al
Forum: Pacific Biosciences 07-27-2015, 05:57 AM
Replies: 12
Views: 5,416
Posted By tacana
Both things, cell being overloaded or having to...

Both things, cell being overloaded or having to much unbound polymerase, can cause the phenotype that you show. Have you tried lowering the concentration of your DNA? I think that this is a first...
Forum: Pacific Biosciences 05-30-2014, 07:15 AM
Replies: 6
Views: 6,246
Posted By tacana
It is difficult to figure out which contaminants...

It is difficult to figure out which contaminants affects the Pacbio polymerase since many of these contaminants can be unique to an organism, growth conditions, DNA extraction method, etc. ...
Forum: Pacific Biosciences 05-23-2014, 08:46 AM
Replies: 5
Views: 4,290
Posted By tacana
Hi Jeff, I sent you my answer in a private...

Hi Jeff,
I sent you my answer in a private message. Let me know if you got it.
Forum: Pacific Biosciences 05-23-2014, 07:34 AM
Replies: 5
Views: 4,290
Posted By tacana
Hello Jeffrey, We have also had many instances...

Hello Jeffrey,
We have also had many instances in which our libraries look good but the results did not reflect that. How did the ZMW distribution looked?

In our case many of these samples had...
Forum: Pacific Biosciences 05-13-2014, 09:26 AM
Replies: 6
Views: 6,246
Posted By tacana
From the information that you show, to me it...

From the information that you show, to me it looks like your cell was a bit underloaded. A P0 means that the instrument is not able to detect any signal on that ZMW. There are several reasons for...
Forum: Pacific Biosciences 09-25-2013, 08:37 AM
Replies: 14
Views: 7,565
Posted By tacana
Sorry for the confusion that I may have cause. ...

Sorry for the confusion that I may have cause. You are correct for 20kb fragments that have been selected with Pippin we also load 150pM -250pm. For the data that I posted, which was not Pippin...
Forum: Pacific Biosciences 06-11-2013, 05:41 AM
Replies: 14
Views: 7,565
Posted By tacana
At least for us, we just started using Blue...

At least for us, we just started using Blue Pippin for size selections.
In regards to loading it seems to vary from library to library but for large libraries (>10kb) we have gone as high as...
Forum: Pacific Biosciences 02-27-2013, 12:54 PM
Replies: 14
Views: 7,565
Posted By tacana
Our results are similar to what flxlex reports. ...

Our results are similar to what flxlex reports. We have tried XLC2 and XLXL using 1x120 movies and got about 20kb-25kb Maximum Mapped Readlength for the XLC2 and 25-30KB for the XLXL (Maximum Mapped...
Forum: Pacific Biosciences 02-19-2013, 10:32 AM
Replies: 12
Views: 5,416
Posted By tacana
rhall- you mentioned that there is room for...

rhall- you mentioned that there is room for optimization. What would you consider an good loading distribution of ZMWs?
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