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Forum: Ion Torrent 09-12-2018, 10:09 PM
Replies: 4
Views: 5,566
Posted By LMA_2018
Hi, To follow up, we basically ran our...

Hi,
To follow up, we basically ran our original library protocol on the PGM system and there out a good data output - 1.1GB of data!
So a problem with the library construction would only be the...
Forum: Ion Torrent 08-27-2018, 10:45 PM
Replies: 4
Views: 5,566
Posted By LMA_2018
Hi, Just for anyone that wants to know; It...

Hi, Just for anyone that wants to know; It appears that the problem was due to the templating process on the OT2. Our machine broke down with an error '56:58 Dual Amplification error" which was very...
Forum: Ion Torrent 07-19-2018, 07:35 PM
Replies: 4
Views: 5,566
Posted By LMA_2018
Hi r.rosati, Yes they do look like dimers. We...

Hi r.rosati,
Yes they do look like dimers. We run a fusion primer approach to amplify our target. The FAS did mention that there was a problem with our purification process which we tweaked with a...
Forum: Ion Torrent 07-13-2018, 02:17 AM
Replies: 4
Views: 5,566
Posted By LMA_2018
Question Ion S5 troubleshooting

Hi, My lab recently purchased and set up a Ion S5 XL system and after the initial set up and verification runs we began running 400bp fusion primer samples using OneTouch 2 (there was about 2 months...
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