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Forum: The Pipeline 11-08-2012, 09:01 AM
Replies: 80
Views: 252,138
Posted By Scoob

Hmm. My XBox 360 looks a lot like that.....
Forum: Sample Prep / Library Generation 12-13-2011, 04:38 AM
Replies: 3
Views: 3,165
Posted By Scoob
For me the Clontech SMARTer kit worked...

For me the Clontech SMARTer kit worked beautifully. Used 100pg total RNA and generated nice Bioanalyser profiles. Also titrated down from 50 to 1 cell and got good products with all of them. You do...
Forum: Illumina/Solexa 03-21-2011, 03:57 AM
Replies: 45
Views: 27,915
Posted By Scoob
Yes keep us posted. We are trying to decide on...

Yes keep us posted. We are trying to decide on Truseq or Sureselect 50 for deeper exome cover and I'm worried that the reported lower coverage, combined with the greater target area with Truseq might...
Forum: RNA Sequencing 10-12-2010, 02:40 AM
Replies: 1
Views: 2,544
Posted By Scoob
Intron spikes in Nugen Ovation data?

We are seeing very large spikes in RNA seq reads in introns using the Nugen WT Ovation pico prep method. These peaks dominate the read profile even more than exonic reads. As a result reads mapping...
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