SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 14 of 14
Search took 0.00 seconds.
Search: Posts Made By: tmm343
Forum: Sample Prep / Library Generation 04-06-2017, 12:16 PM
Replies: 0
Views: 1,228
Posted By tmm343
Drop-seq vs. Dolomite RNA-seq System

Hello Everyone,

Has anyone used the Dolomite Single cell RNA-seq system? Also can anyone tell me what the difference is between this system and the Drop-seq setup/protocol?

Thank you!
Forum: Sample Prep / Library Generation 10-10-2016, 11:06 AM
Replies: 6
Views: 3,274
Posted By tmm343
We have not found a solution and are picking...

We have not found a solution and are picking these experiments and troubleshooting back up very soon! Will keep all updated.
Forum: Sample Prep / Library Generation 07-20-2016, 08:24 AM
Replies: 6
Views: 3,274
Posted By tmm343
Single Cell RNA-seq Troubleshooting

Hello All,

We are trying to perform single cell RNA-seq in our facility. We would like to do this in a high-throughput manner on our robotic system and are working off of this published protocol:...
Forum: Sample Prep / Library Generation 07-01-2016, 07:29 AM
Replies: 13
Views: 4,125
Posted By tmm343
Hi All, We normally qPCR but for these 16S...

Hi All,

We normally qPCR but for these 16S and 18S libraries we have found Qubit to be more than sufficient and have been using it successfully and consistently and just recently ran into this...
Forum: Sample Prep / Library Generation 06-29-2016, 11:56 AM
Replies: 13
Views: 4,125
Posted By tmm343
Unhappy Quantitation Variations with Qubit

Hello All,

I have run into quite a strange issue recently.

I have successfully and consistently been running 16S and 18S libraries and quantifying the final multiplexed pool using the Qubit....
Forum: Academic/Non-Profit Jobs 12-10-2014, 08:03 AM
Replies: 0
Views: 1,093
Posted By tmm343
NYU CGSB Genomics Core Facility Technician Position

The NYU Center for Genomics and Systems Biology seeks a full-time laboratory technician to assist in the Genomics Core Facility operations. The Core Facility supports multiple projects and research...
Forum: Illumina/Solexa 12-04-2014, 10:07 AM
Replies: 8
Views: 2,175
Posted By tmm343
GenoMax - I have been working with tech support...

GenoMax - I have been working with tech support and they believe it is a primer or library issue most likely but aren't 100% sure. I find both a little hard to believe since it is the same issue in...
Forum: Illumina/Solexa 12-04-2014, 08:17 AM
Replies: 8
Views: 2,175
Posted By tmm343
The custom primers are different between the runs...

The custom primers are different between the runs and from two separate labs. One set is straight from the Caparoso paper for 16S sequencing. The other set has been ordered by the lab but same...
Forum: Illumina/Solexa 12-04-2014, 07:07 AM
Replies: 8
Views: 2,175
Posted By tmm343
MiSeq 500 Cycle Kit - Low Read 2 Qualities

Very specific question but has anyone encountered low read quality in only read 2 on the MiSeq using a 500 cycle v2 kit with run configuration of 2x250?

I have does 3 consecutive runs now with...
Forum: Illumina/Solexa 11-17-2014, 06:11 AM
Replies: 9
Views: 1,981
Posted By tmm343
1) Cluster density and if they are within range...

1) Cluster density and if they are within range for the SBS chemistry and machine.
The cluster density looks low as reported by SAV. 400-600 range.

2) If they are within range or lower, check...
Forum: Illumina/Solexa 11-14-2014, 08:10 AM
Replies: 9
Views: 1,981
Posted By tmm343
nucacidhunter, The samples are RNA-seq...

nucacidhunter,
The samples are RNA-seq libraries so I assume after this initial 6bp barcode the base composition should be pretty diverse. My overal raw reads was extremely low as well even while I...
Forum: Illumina/Solexa 11-12-2014, 12:55 PM
Replies: 9
Views: 1,981
Posted By tmm343
Unfortunately I only have the first 6 bases as...

Unfortunately I only have the first 6 bases as these are the barcode sequences I was provided with. The data has yet to be analyzed so I do not have the following base composition.
Forum: Illumina/Solexa 11-11-2014, 06:33 AM
Replies: 9
Views: 1,981
Posted By tmm343
HiSeq Low Read Count - Barcode in Read1

Hello All,

I have a customer with their barcode at the very beginning of their read 1. I consistently am seeing a low number of raw reads as well as those PF. I am told it is a base diversity...
Forum: Academic/Non-Profit Jobs 11-05-2014, 01:20 PM
Replies: 0
Views: 1,002
Posted By tmm343
Clinical Bioinformatics Assistant Professor Position at NYU

New York University invites applications for a Clinical Assistant Professor appointment in the Department of Biology to start September 1, 2015 pending budgetary and administrative approval....
Showing results 1 to 14 of 14

 


All times are GMT -8. The time now is 05:06 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO