SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 9 of 9
Search took 0.00 seconds.
Search: Posts Made By: Myrmex
Forum: Sample Prep / Library Generation 10-13-2015, 06:23 AM
Replies: 9
Views: 2,388
Posted By Myrmex
Thanks for all the help guys! This is a little...

Thanks for all the help guys! This is a little pricier then we thought it was going to be, so we are figuring out the funds...
Forum: Sample Prep / Library Generation 10-12-2015, 09:00 AM
Replies: 9
Views: 2,388
Posted By Myrmex
Hi, thanks for getting back to me—we are at the...

Hi, thanks for getting back to me—we are at the Chicago Botanic Garden. Am I correct in understanding that the price is $220 per sample but you need at least 24 samples to get that price?
Forum: Sample Prep / Library Generation 10-12-2015, 06:34 AM
Replies: 9
Views: 2,388
Posted By Myrmex
Are there any sub $200 library prep services for RNAseq?

We are looking at doing RNAseq on around 36 samples we have extracted and want to outsource the library prep. I have shopped around a little and everything seems to be above $200. Does anyone know of...
Forum: Sample Prep / Library Generation 03-04-2015, 12:11 PM
Replies: 17
Views: 4,156
Posted By Myrmex
I did run undiluted controls a couple of times...

I did run undiluted controls a couple of times but should start doing it every time. If I run undiluted controls over and over (without changing anything) there variation of maybe around 5–20% or so...
Forum: Sample Prep / Library Generation 03-04-2015, 09:29 AM
Replies: 17
Views: 4,156
Posted By Myrmex
Okay, that makes a lot of sense. Thanks so much...

Okay, that makes a lot of sense. Thanks so much for your help everyone, we've been struggling with this for a while. It's nice to have something else to go off of.
Forum: Sample Prep / Library Generation 03-03-2015, 02:20 PM
Replies: 17
Views: 4,156
Posted By Myrmex
okay thank you very much—I will go from there......

okay thank you very much—I will go from there... Do you have any feeling for whether or not I should be concerned about DNA loss with filter extraction, or filter cleanups? I know that is a common...
Forum: Sample Prep / Library Generation 03-03-2015, 12:49 PM
Replies: 17
Views: 4,156
Posted By Myrmex
Okay, thank you, this makes a lot of sense. We do...

Okay, thank you, this makes a lot of sense. We do have viscous material in our CTAB extractions which is one of the ideas we came up with for why this is happening. When I decrease the volume of the...
Forum: Sample Prep / Library Generation 03-02-2015, 12:48 PM
Replies: 17
Views: 4,156
Posted By Myrmex
Thanks for the suggestion. We haven't run gels...

Thanks for the suggestion. We haven't run gels yet. I don't have any experience running gels for quantification but have been reading about it. That seems like a good side check that the input...
Forum: Sample Prep / Library Generation 03-02-2015, 11:07 AM
Replies: 17
Views: 4,156
Posted By Myrmex
Cannot Normalize DNA for GBS/RadSeq, Nonsense Qbit results

Our lab is carrying out an initial pilot test of two different RADseq/GBS protocols to see which will work best for us. Both protocols emphasize the importance of normalizing DNA extractions before...
Showing results 1 to 9 of 9

 


All times are GMT -8. The time now is 06:53 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO