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Forum: RNA Sequencing 11-24-2020, 11:18 AM
Replies: 0
Views: 1,513
Posted By dlhmmll
NGS result that can't be verified with RT-PCR

I received 5 plant leaf samples for NGS. Two of the 5 were determined to be positive for virus via RT-PCR. However when I performed NGS, all 5 samples were determined to be positive. In the 3 samples...
Forum: Illumina/Solexa 08-22-2018, 03:42 AM
Replies: 5
Views: 1,633
Posted By dlhmmll
Thanks for the replies

Thanks for the replies
Forum: Illumina/Solexa 08-21-2018, 10:59 AM
Replies: 5
Views: 1,633
Posted By dlhmmll
Correction: I mean "undetermined"

Correction: I mean "undetermined". I usually don't spike my runs with PhiX either
Forum: Illumina/Solexa 08-21-2018, 10:54 AM
Replies: 5
Views: 1,633
Posted By dlhmmll
Unidentified Reads in Base Calls

When looking for my .fasta files under MiSeq Analysis > Data > Base Calls, there are always small .fasta files called "unidentified". Why do I get these, what are they, and are they useful? I'm doing...
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