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Forum: Sample Prep / Library Generation 12-07-2016, 11:57 AM
Replies: 4
Views: 2,031
Posted By seqgirl123
Hello, I have found a paper here...

Hello,

I have found a paper here http://www.nature.com/nmeth/journal/v11/n2/full/nmeth.2772.html that the supplementary table 2 "list of custom oligonucleotides" they published the full list of...
Forum: Sample Prep / Library Generation 12-05-2016, 05:49 AM
Replies: 4
Views: 2,031
Posted By seqgirl123
cmbetts, thank you for your reply. I will be...

cmbetts, thank you for your reply. I will be adding ERCC to the lysis mix in single cells. I prefer ERCC too. Do you know where I can find how to dilute ERCC into single cell lysis reactions?
...
Forum: Sample Prep / Library Generation 12-03-2016, 10:32 AM
Replies: 4
Views: 2,031
Posted By seqgirl123
Smart-Seq v4 ultra low RNA kit

Hello,

I would be using this kit for single cell RNA in a 96 well plate. I will be preparing the reactions in a plate. I would like to know if anyone has spiked in ERCC or Array Control RNA...
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