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Forum: Sample Prep / Library Generation 05-19-2015, 07:11 AM
Replies: 20
Views: 6,546
Posted By Jfly7
Thanks for the reply. I am doing genomic DNA...

Thanks for the reply.
I am doing genomic DNA library prep and am using HiSeq 2500 100bp PE reads. The reason I want libraries a bit longer than what I got is because a lot of fragments were short...
Forum: Sample Prep / Library Generation 05-19-2015, 06:13 AM
Replies: 20
Views: 6,546
Posted By Jfly7
Hi Amelia, I found this question and I am...

Hi Amelia,
I found this question and I am wondering if you had any luck making adjustments to make longer libraries with Nextera? I prepared libraries with Nextera last year and also got shorter...
Forum: Bioinformatics 05-04-2015, 06:15 AM
Replies: 1
Views: 744
Posted By Jfly7
Program that gives summary of substitutions

Hey,

I am looking for a program that gives a summary of base substitutions from a vcf file, e.g. number of A --> T, C --> T, etc. substitutions. Anyone know of a program that can do this so I...
Forum: Bioinformatics 12-15-2014, 05:54 PM
Replies: 0
Views: 1,406
Posted By Jfly7
How to get allele frequency histogram from vcf file?

Hi Everyone,

I am new to SEQanswers and the bioinformatics world, and I am looking for some help. I would like to get a count of allele frequencies in bins from a VCF file (i.e. to create a...
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