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Forum: Bioinformatics 12-24-2014, 07:37 AM
Replies: 9
Views: 2,643
Posted By GenoMax
Use the following command to find out what tags...

Use the following command to find out what tags are represented in your "undetermined" file. You will generally see a great variation present. Thing to look for is if there are some tags that are way...
Forum: Bioinformatics 12-24-2014, 07:27 AM
Replies: 9
Views: 2,643
Posted By GenoMax
What fraction of the reads are in the...

What fraction of the reads are in the undetermined file?

Are you recovering all the samples you expect from the run? A common problem is specifying an incorrect barcode for a sample which sends...
Forum: Bioinformatics 12-24-2014, 06:50 AM
Replies: 9
Views: 2,643
Posted By GenoMax
Can you specify what kind of "trouble" you ran...

Can you specify what kind of "trouble" you ran into with bcl2fastq?

Please keep in mind that if there was a problem with the index reads (multiple N's in the sequence) then no demultiplexing tool...
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