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Search: Posts Made By: MadsAlbertsen
Forum: Bioinformatics 01-23-2012, 12:52 AM
Replies: 4
Views: 524
Posted By MadsAlbertsen
Most assemblers have a function to obtain the...

Most assemblers have a function to obtain the average coverage for each contig - it's a long time since I've used ABySS so I can't remember if it has an output contig coverage option.

Otherwise...
Forum: Bioinformatics 01-22-2012, 05:21 PM
Replies: 4
Views: 524
Posted By MadsAlbertsen
The first thing I do after an assembly is always...

The first thing I do after an assembly is always a contig length vs. coverage plot to estimate the repeat content. It's usually quite easy to see the repeats.

rgds
Mads
Forum: Bioinformatics 12-22-2011, 11:32 PM
Replies: 5
Views: 1,461
Posted By MadsAlbertsen
We've done anything from 200x-2000x (depending on...

We've done anything from 200x-2000x (depending on if we can fill the machine..) and I do not see much difference in the assembly above 300x.

We do not use matepairs normally as we are rarely...
Forum: Bioinformatics 12-22-2011, 01:43 AM
Replies: 5
Views: 1,461
Posted By MadsAlbertsen
I would do PE 2x100 and a Matepair of 5Kb. ...

I would do PE 2x100 and a Matepair of 5Kb.

In my experience it is atleast as good if not better than 454 (only done 1x454 genome..).

rgds
Mads Albertsen
Forum: Bioinformatics 06-03-2011, 11:38 AM
Replies: 7
Views: 1,220
Posted By MadsAlbertsen
I would go with a small/medium sized cluster and...

I would go with a small/medium sized cluster and then go for large jobs in the cloud instead.

From my point of view it is way too expensive to scale the computer needs after the peak requirements...
Forum: Bioinformatics 05-26-2011, 11:01 AM
Replies: 9
Views: 1,648
Posted By MadsAlbertsen
I would not try to assemble the data at all. 200k...

I would not try to assemble the data at all. 200k 454 reads seems very low to get any decent assembly even in very simple communities (or even in single genomes).

200.000 reads x 250 bp read...
Forum: De novo discovery 05-02-2011, 08:10 AM
Replies: 8
Views: 2,123
Posted By MadsAlbertsen
I've been doing this to manually close gaps that...

I've been doing this to manually close gaps that can't be assembled using various short read assemblers and it generally works great if you restrict the new denovo assembly to the regions where you...
Forum: RNA Sequencing 04-15-2011, 05:32 AM
Replies: 3
Views: 979
Posted By MadsAlbertsen
There are basically two possible problems: ...

There are basically two possible problems:

1) The removal efficiency is highly dependant on intact rRNA. Make sure that rRNA is not degraded before applying MICROBExpress.

Working faster and...
Forum: Bioinformatics 10-23-2010, 01:38 AM
Replies: 7
Views: 1,929
Posted By MadsAlbertsen
Have you considered using the USEARCH package...

Have you considered using the USEARCH package instead of BLAST? It might make it possible to do large scale database search?

rgds
MA
Forum: Bioinformatics 10-20-2010, 10:13 PM
Replies: 7
Views: 1,929
Posted By MadsAlbertsen
You should check: A human gut microbial gene...

You should check: A human gut microbial gene catalogue established by metagenomic sequencing. (doi:10.1038/nature08821)

To my knowledge this is the only study who have published large scale...
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