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Forum: RNA Sequencing 03-04-2010, 04:43 AM
Replies: 155
Views: 61,262
Posted By steffenp
Hi, I wanted to use DEGseq to identify...

Hi,
I wanted to use DEGseq to identify differentially expressed genes between wildtype and mutant experiments. I have 4 datasets for each condition (WT,mutant) : 2 biological replicates and for each...
Forum: Bioinformatics 01-15-2010, 03:55 AM
Replies: 52
Views: 82,346
Posted By steffenp
RPKM for Tag-Seq data

Hello everyone!
My question is also related to the RPKM normalization. Is this measure suited for Tag-Seq. data, where we have only reads at the 3'end and not all over the whole transcript. My...
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