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Forum: The Pipeline 03-02-2015, 05:47 AM
Replies: 16
Views: 14,088
Posted By larissa
Thanks AllSeq for the info! Btw, your knowledge...

Thanks AllSeq for the info! Btw, your knowledge base is quite helpful.
Forum: The Pipeline 02-25-2015, 09:43 AM
Replies: 16
Views: 14,088
Posted By larissa
I was not sure if to post this here or in the...

I was not sure if to post this here or in the Solid forum, but I figured this would work too, so anyone knows what is going on (if anything) with the Solid platform? How commonly is it (still) used?
Forum: The Pipeline 02-21-2012, 05:57 AM
Replies: 51
Views: 182,940
Posted By larissa
That's one of the reasons why 4% error rate is...

That's one of the reasons why 4% error rate is not acceptable in the clinic, for guiding treatment/dosing and inclusion/exclusion criteria. It will be acceptable for lots of other things, all R&D...
Forum: The Pipeline 02-17-2012, 11:21 AM
Replies: 103
Views: 139,743
Posted By larissa
Oxford Nanopore’s shareholders include IP Group...

Oxford Nanopore’s shareholders include IP Group Plc, which owns 21.5 percent, hedge-fund manager Lansdowne Partners LP and Invesco Perpetual, the U.K. group of mutual funds. The company also has...
Forum: The Pipeline 02-17-2012, 11:10 AM
Replies: 51
Views: 182,940
Posted By larissa
They better do something with Roche coming after...

They better do something with Roche coming after them.
Forum: The Pipeline 02-17-2012, 10:46 AM
Replies: 51
Views: 182,940
Posted By larissa
So, doesn't Illumina have stakes in ONT?

So, doesn't Illumina have stakes in ONT?
Forum: The Pipeline 02-17-2012, 10:40 AM
Replies: 51
Views: 182,940
Posted By larissa
Even with 4% error rates? Will they deliver in...

Even with 4% error rates? Will they deliver in improving that? That's not acceptable for clinical use. It may be very useful for a lot of other stuff.
Forum: Illumina/Solexa 08-16-2011, 10:42 AM
Replies: 7
Views: 89,290
Posted By larissa
Something like this? Citation: Neiman M, Lundin...

Something like this?
Citation: Neiman M, Lundin S, Savolainen P, Ahmadian A (2011) Decoding a Substantial Set of Samples in Parallel by Massive Sequencing. PLoS ONE 6(3): e17785....
Forum: Ion Torrent 08-10-2011, 06:25 PM
Replies: 18
Views: 21,563
Posted By larissa
Could someone please clarify what is the referred...

Could someone please clarify what is the referred guava for the Ion? Because I am exactly going through protocols to tally up everything needed to set up the system and do not remember reading about...
Forum: RNA Sequencing 03-18-2010, 05:07 AM
Replies: 19
Views: 9,510
Posted By larissa
Hi Jay, I like this one: ...

Hi Jay, I like this one:
http://www.bioinfo.rpi.edu/zukerm/cgi-bin/rna-index.cgi
It gives a lot of info, more than i really can understand. You can use it for DNA or RNA and play around with...
Forum: Sample Prep / Library Generation 03-17-2010, 09:11 AM
Replies: 15
Views: 5,680
Posted By larissa
Do not know, sorry... Have you tried Illumina...

Do not know, sorry... Have you tried Illumina tech support? I have had good experiences in the past with them.
Forum: Sample Prep / Library Generation 03-17-2010, 08:38 AM
Replies: 15
Views: 5,680
Posted By larissa
Hi Ben and Phillip, I just came across a review...

Hi Ben and Phillip,
I just came across a review on Current Protocols in Molecular Biology 2010 (RNA-Seq: A Method for Comprehensive Transcriptome Analysis by Nagalakshmi et al). This is specific for...
Forum: Sample Prep / Library Generation 03-16-2010, 11:32 AM
Replies: 15
Views: 5,680
Posted By larissa
Hi, maybe this can help a bit. As I recall,...

Hi, maybe this can help a bit.
As I recall, Illumina only recommends checking the library on the bioanalyzer after the final enrichment/PCR step. Perhaps you should not expect to see anything at...
Forum: Sample Prep / Library Generation 03-16-2010, 11:21 AM
Replies: 26
Views: 29,333
Posted By larissa
Hello peromhc, I do not have much experience with...

Hello peromhc, I do not have much experience with the sequencing part but feel comfortable in commenting on the bioanalyzer trace. I would not expect such a sharp peak as result of degradation....
Forum: RNA Sequencing 03-16-2010, 06:57 AM
Replies: 19
Views: 9,510
Posted By larissa
Hi, new here so please bare with me and any...

Hi, new here so please bare with me and any mistakes. Have you checked the article by Mortazavi et all - Mapping and quantifying mammalian transcriptomes by RNA-Seq, Nature Methods 2008 v.5(7): 621....
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