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Forum: 454 Pyrosequencing 05-25-2011, 12:26 AM
Replies: 0
Views: 1,561
Posted By elly
Smile GS FLX Mate Paired End library Construction with MID

Is there anyone who have any information about "GS FLX Mate
Paired End library construction with MID"?
Forum: Illumina/Solexa 12-01-2008, 07:20 PM
Replies: 3
Views: 3,883
Posted By elly
Homemade illumina oligos and library prep kit

Hi,

I am wondering if we can use the PCR amplification primers which were made at our side.
In theory, this can work properly.

But I am worrying a little bit if there can be some problem with...
Forum: Sample Prep / Library Generation 08-02-2008, 01:18 AM
Replies: 18
Views: 12,650
Posted By elly
Hi kmay, I'm so sorry that I didn't reply to...

Hi kmay,

I'm so sorry that I didn't reply to my thread.
I was so busy to set up another application.

I'm sure that my referernce genome sequence was correct.

Could you please explain why...
Forum: 454 Pyrosequencing 06-17-2008, 07:20 AM
Replies: 2
Views: 2,484
Posted By elly
High Short Reads %

Hello, there,,

Recently, I perfomed the GS FLX Sequencing run using 70X75 PTP, 4 Regions Gasket.
Unfortunately, I got some weired results.
Actual keypass reads# of my library was about ~170000,...
Forum: Sample Prep / Library Generation 04-15-2008, 09:10 PM
Replies: 18
Views: 12,650
Posted By elly
ChIP-Seq problems with library generation

I am trying to sequencing ChIPed DNA.
My DNA was sonicated to 500~1kbp during IP step.
I chose two ways to solve this size problem.
Before starting library preparation for SOLEXA, one was...
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