Forum: Sample Prep / Library Generation
07-03-2013, 07:25 AM
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Replies: 1
Views: 1,343
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Forum: Sample Prep / Library Generation
10-02-2012, 02:18 PM
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Replies: 17
Views: 7,423
Size selection beyond XP bead ability!
Hi, Philip,
It is very difficult or impossible using XP bead for size selection of NA. especially for small and precise NA at specific bp/nt. Also got same inoformation from Beckman Coulter.
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Forum: Sample Prep / Library Generation
10-01-2012, 06:45 PM
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Replies: 17
Views: 7,423
Ok,
we have three method for purification or...
Ok,
we have three method for purification or concentrate the NA,
1) precipitation
2) bead
3) column
precipitation will get the highest yield ratio, but time consumable, at lest 1hr.
1) 50ul...
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Forum: Sample Prep / Library Generation
09-28-2012, 07:23 PM
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Replies: 17
Views: 7,423
Hi, Phillip,
I design this method base on...
Hi, Phillip,
I design this method base on properties of some chaotropics, like Ethanol, isopropanol,.. selection of those chaotropics let us change the range of purity flexible. for save beads, for...
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Forum: Sample Prep / Library Generation
09-26-2012, 10:56 PM
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Replies: 17
Views: 7,423
Hi, Everyone,
I think for cleanup RNA small...
Hi, Everyone,
I think for cleanup RNA small fragment just try XP bead-RNase free,
1. if you have 100ul RNA solution, add:
(1) 3M NaOAc 25ul to final contration 0.3M
(2) Isopropanol...
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Forum: Sample Prep / Library Generation
09-06-2012, 09:50 PM
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Replies: 3
Views: 2,681
Hi, Anjani,
i do not know what kind of your...
Hi, Anjani,
i do not know what kind of your library, i try to contribute some minds.Ok, Agencourt AMPure beads is excellent product for purefication for DNA and RNA, they have RNA Clean beads for...
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Forum: Sample Prep / Library Generation
09-06-2012, 09:23 PM
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Replies: 1
Views: 1,463
Hi,
I am not sure Illumina RNA seq library...
Hi,
I am not sure Illumina RNA seq library length and concentration. but i am sure the SOLiD RNA library some information. After your PCR and purification process, you will get several ng library,...
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Forum: SOLiD
08-15-2012, 08:41 PM
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Replies: 2
Views: 2,031
As my calculate, i think that is true,...
As my calculate, i think that is true, 10millinon/ul, EZ80 usually use 1430ul x 10millions. method should be same like IKA utilized, which apply 160ul same concentration beads. AB maybe give you more...
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Forum: 454 Pyrosequencing
08-14-2012, 07:00 PM
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Replies: 3
Views: 1,606
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Forum: SOLiD
08-14-2012, 06:36 PM
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Replies: 0
Views: 1,368
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