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Forum: 454 Pyrosequencing 09-17-2012, 11:28 PM
Replies: 0
Views: 1,413
Posted By aperdomos
Large No. small fragments Deep Amplicon Seq (454-GS-FLX)-demultiplexing problems

Hello everyone,

I just got the results of my Deep Amplicon sequencing project (fusion primer) from my sequencing service provider (platform used: Roche GS-FLX). As you can see below (***), the...
Forum: Bioinformatics 06-18-2012, 06:31 PM
Replies: 2
Views: 1,496
Posted By aperdomos
Many thanks for your answer and additional...

Many thanks for your answer and additional information.

Re to the aligners, I'm planning to use two different "BWA and Mosaik".

Tks again!!
Forum: Bioinformatics 06-18-2012, 07:46 AM
Replies: 2
Views: 1,496
Posted By aperdomos
From SAM/BAM to FASTQ- re-mapping possibilities?

Dear All,

I hope not to be inopportune with this e-mail, which initially was sent as private message to "maubp". I’m new novice in the world of NGS data sets analysis. I've reading many posts...
Forum: Bioinformatics 01-30-2012, 03:35 PM
Replies: 4
Views: 1,829
Posted By aperdomos
kentk and SES

Many thanks for the info.
Forum: Bioinformatics 01-24-2012, 07:46 AM
Replies: 4
Views: 1,829
Posted By aperdomos
Does anyone know about some future "close" NGS workshop

Hello everyone,

As a beginner in the world of NGS, I'm requiring some training on NGS. Therefore, I want to know if anyone knows about some future "close" workshop about NGS data analyses:...
Forum: Bioinformatics 01-24-2012, 07:41 AM
Replies: 1
Views: 1,786
Posted By aperdomos
How to subtract specific DNA sequences from *mpileup* files (programs and scripts)

Hello everyone,

I have around 100 mpileup files from different species. These files have some specific DNA regions that are of my interest, since they include some genes that I want to compare....
Forum: Bioinformatics 01-19-2012, 01:25 AM
Replies: 2
Views: 1,424
Posted By aperdomos
Swbarnes2. Many thanks for your answer; it...

Swbarnes2.

Many thanks for your answer; it helped me to clarify some of my concerns. I was using the text editor to explore the files (pileups); however, the big number of samples from different...
Forum: Bioinformatics 01-18-2012, 06:32 PM
Replies: 2
Views: 1,424
Posted By aperdomos
How to analyse and visualize “vcrf” files (*vcrfpileup; *pileup*) with samtools-other

Hello everyone,

I got some files with DNA sequencing preliminary data from a partner lab. Before they sent me these data, they were processed using variant calling with the samtools pileup option...
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