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Forum: Epigenetics 06-17-2020, 02:55 AM
Replies: 0
Views: 511
Posted By kpet
does heat-treatment (for covid inactivation) of Oragene saliva DNA kits alter DNA met

Has anyone heat-treated Oragene DNA saliva collection kits and done downstream DNA methylation analysis on the extracted DNA? Any evidence of an effect on DNA methylation?

While DNA Genotek say...
Forum: Sample Prep / Library Generation 03-04-2020, 03:18 AM
Replies: 0
Views: 863
Posted By kpet
does the Oragene saliva kit lyse coronavirus?

Does anybody know if the Oragene DNA saliva collection kits(s) lyse coronavirus?
Forum: Core Facilities 12-02-2019, 09:30 PM
Replies: 2
Views: 2,038
Posted By kpet
EPIC arrays

EPIC arrays
Forum: Core Facilities 12-02-2019, 01:18 AM
Replies: 2
Views: 2,038
Posted By kpet
post-bisulphite conversion QC check

Does anyone know of a quicker alternative to PCR/agarose gel when QCing bisulphite conversion in large numbers of samples?
Forum: General 11-28-2019, 07:37 AM
Replies: 0
Views: 2,025
Posted By kpet
DNA extraction from buccal cells - any favourite methods?

I'm looking into methods for extracting DNA from buccal cells. Does anyone do this? What method to do you use and why do you use it?
Thanks!
Forum: Oxford Nanopore 10-24-2019, 01:29 AM
Replies: 2
Views: 3,713
Posted By kpet
Thanks - I was hoping that was the case. It'd...

Thanks - I was hoping that was the case. It'd probably be no more than an hour.
Forum: Oxford Nanopore 10-18-2019, 07:26 AM
Replies: 2
Views: 3,713
Posted By kpet
can I use standard plastic falcon tubes for chloroform DNA extraction?

Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative impact...
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