Forum: Illumina/Solexa
07-23-2015, 03:44 AM
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Replies: 6
Views: 3,203
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Forum: Illumina/Solexa
07-23-2015, 03:20 AM
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Replies: 6
Views: 3,203
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Forum: Illumina/Solexa
07-22-2015, 06:45 AM
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Replies: 6
Views: 3,203
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Forum: Illumina/Solexa
01-26-2015, 01:01 AM
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Replies: 53
Views: 89,174
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Forum: Illumina/Solexa
01-25-2015, 09:43 AM
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Replies: 53
Views: 89,174
There should be an exact max of clusters for...
There should be an exact max of clusters for HiSeq 3000/4000 defined by the number of microwells (325M as stated above?).
We're getting regularly 300M reads on the upgraded 2500. If 325M is correct,...
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Forum: Illumina/Solexa
01-22-2015, 11:47 PM
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Replies: 53
Views: 89,174
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Forum: Core Facilities
09-26-2014, 03:06 AM
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Replies: 1
Views: 2,865
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Forum: Illumina/Solexa
12-04-2013, 02:15 AM
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Replies: 18
Views: 8,273
Our local sales representative just explained...
Our local sales representative just explained that the 2x250 upgrade is independent from the 1Tb upgrade.
One of our HiSeqs was upgraded this year to 2500 but it seems that the camera is still too...
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Forum: Illumina/Solexa
07-04-2013, 12:57 AM
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Replies: 9
Views: 3,514
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Forum: Sample Prep / Library Generation
02-18-2013, 12:49 AM
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Replies: 3
Views: 1,546
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Forum: Sample Prep / Library Generation
02-13-2013, 03:31 AM
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Replies: 3
Views: 1,546
Cheapest 96 well WGS sample prep
Hi,
Has anybody tried to minimize sample prep costs (for Illumina sequencing) in 96 well format? How low have you got and using which reagents?
We want to sequence >20k samples and while heavily...
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Forum: Sample Prep / Library Generation
02-28-2012, 03:06 AM
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Replies: 1
Views: 1,572
Bioanalyzer peak at 107bp
Hi,
we're getting a narrow single peak at 107bp after size selection on gel and subsequent PCR.
Apart from that, bioanalyzer profiles of the libraries look fine.
I'm pretty sure there have been...
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Forum: Illumina/Solexa
11-22-2011, 08:46 AM
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Replies: 15
Views: 6,172
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Forum: Illumina/Solexa
11-22-2011, 06:43 AM
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Replies: 15
Views: 6,172
We had to fill up a PE flowcell and took...
We had to fill up a PE flowcell and took therefore this SR library (small RNA adapters no Truseq).
It didn't really work, very few, very small clusters. I was expecting to get the first read only...
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Forum: Illumina/Solexa
11-21-2011, 07:06 AM
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Replies: 15
Views: 6,172
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Forum: Illumina/Solexa
11-21-2011, 03:06 AM
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Replies: 15
Views: 6,172
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Forum: Sample Prep / Library Generation
08-10-2011, 08:17 AM
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Replies: 2
Views: 14,308
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Forum: Sample Prep / Library Generation
08-10-2011, 07:45 AM
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Replies: 2
Views: 14,308
Truseq Multiplexing / Minimal number of Samples
Hi,
we have been working for a while with custom barcoded adapters. Successful basecalling seems to depend mostly on sufficient base complexity and we therefore run a minimum of 4 samples per...
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Forum: Pacific Biosciences
05-10-2011, 07:22 AM
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Replies: 1
Views: 3,491
Hands on experience someone?
Hi,
I have still only little information on how PacBio's system is performing. Anyone here who could share some experiences?
Best
AB
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Forum: Illumina/Solexa
09-25-2010, 04:54 AM
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Replies: 7
Views: 4,889
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Forum: Illumina/Solexa
09-25-2010, 04:18 AM
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Replies: 7
Views: 4,889
Nextera says bird-nesting isn't a problem: ...
Nextera says bird-nesting isn't a problem:
"After the Nextera tagmentation reaction and PCR, you may notice an apparent fragment size of 500 bases (or greater) when the DNA is analyzed on an Agilent...
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Forum: Illumina/Solexa
09-23-2010, 07:33 AM
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Replies: 7
Views: 2,202
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Forum: Illumina/Solexa
09-23-2010, 07:24 AM
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Replies: 7
Views: 4,889
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Forum: Illumina/Solexa
09-14-2010, 01:28 AM
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Replies: 7
Views: 2,202
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Forum: Illumina/Solexa
09-09-2010, 05:16 AM
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Replies: 7
Views: 2,202
Erratic cluster numbers
Hi,
Since ~ 2 months we are getting erratic cluster numbers on our GAs (cBot) even if we check the libraries with RT-PCR. Before we were using 7pM (~300.000clusters /tile) and were getting...
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